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Journal of Cluster Science

, Volume 28, Issue 4, pp 2057–2073 | Cite as

Intracellular Uptake of PEG-Funtionalized Baicalein Loaded Iron Oxide Nanoparticles Regulates Apoptotic Genes in Triple Negative Breast Cancer Cells: Mitochondrial Pathway Targeted Therapy for Breast Cancer

  • Krishnamoorthy Kavithaa
  • Sundaravadivelu Sumathi
  • Palghat Raghunathan Padma
Original Paper

Abstract

The recent focus and development of nanotechnology in medicine is countless, which involves diagnostic, therapeutic and preventive systems for various diseases. Nanoparticles have received much attention due to their uses in cancer therapy. The current study focused on the synthesis of baicalein loaded iron oxide nanoparticles and their efficacy against triple negative breast cancer (TNBC) MDA-MB-231. The electron microscopic analysis reveals that the particles were internalized with the various sub cellular regions of selected cancer cells. Further flow cytometric analysis of mitochondrial membrane potential using JC-1 staining showed that significant aggregates were found in the cells treated with baicalein loaded iron oxide nanoparticles, which, in turn, implies momentous mitochondrial membrane potential loss that occurs. Similarly apoptotic and anti-apoptotic gene expression pattern showed that baicalein loaded iron oxide nanoparticles were upregulates the apoptotic genes like Bad, Bax, GADD45 and PARP cleavage in a dose dependant manner. Detailed kit based flowcytometric analysis also reveals that the above findings were significant in the focused field, it is apparent that nano conjugates have the ability to induce apoptosis, DNA damage and cell cycle arrest and decrease the rate of cell proliferation in TNBC cells.

Keywords

Triple negative breast cancer Gene regulation Iron oxide nanoparticles Cellular internalization Flowcytometry JC-1 mitochondrial pathway 

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Department of Biochemistry, Biotechnology and BioinformaticsAvinashilingam Institute for Home Science and Higher Education for Women UniversityCoimbatoreIndia

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