Evaluation of Some Biosynthesized Silver Nanoparticles for Biomedical Applications: Hydrogen Peroxide Scavenging, Anticoagulant and Thrombolytic Activities
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The present study examines the hydrogen peroxide scavenging, anticoagulant and thrombolytic activities of silver nanoparticles (AgNPs) that were biosynthesized using extracts obtained from spider cobweb (CB), pod (KP), seed (KS) and seed shell (KSS) of kolanut (Cola nitida). The nearly spherical shaped AgNPs, with surface plasmon resonance of 431.5–457.5 nm, were polydispersed having sizes of 3–50, 12–80, 8–50, and 5–40 nm for CB, KP, KS and KSS-AgNPs respectively. Hydrogen peroxide scavenging activities of 77–99.8% were obtained using 1–20 µg/ml of AgNPs. The particles prevented the coagulation of blood, and also showed thrombolytic activities of 55.76–89.83%, with KSS-AgNPs having the highest activity. Microscopic examination of the lyzed blood clot supported the thrombolytic activities. On the other hand, silver nitrate solution showed negligible activity of 1.92%, while thrombolysis of 7.55, 8.70, 8.93 and 30.19% were obtained for the extracts of KSS, CB, KS and KP respectively. The results herein presented showed potential biomedical applications of the biosynthesized AgNPs to scavenge free radicals and for the management of blood coagulation disorders and thrombotic diseases.
KeywordsBiosynthesis Silver nanoparticles Anticoagulant activity Thrombolytic activity Blood coagulation disorders
The provision of some facilities used in this investigation by the authority of LAUTECH, Ogbomoso, Nigeria is grateful acknowledged by A. Lateef.
- 7.A. Lateef, S. A. Ojo, A. S. Akinwale, L. Azeez, E. B. Gueguim-Kana, and L. S. Beukes (2015). Biogenic synthesis of silver nanoparticles using cell-free extract of Bacillus safensis LAU 13: antimicrobial, free radical scavenging and larvicidal activities. Biologia 70, 1295–1306.Google Scholar
- 14.I. A. Adelere and A. Lateef (2016). A novel approach to the green synthesis of metallic nanoparticles: the use of agro-wastes, enzymes and pigments. Nanotechnol. Rev. 5, 567–587.Google Scholar
- 15.A. Lateef, S. A. Ojo, and J. A. Elegbede (2016). The emerging roles of arthropods and their metabolites in the green synthesis of metallic nanoparticles. Nanotechnol. Rev. 5, 601–622.Google Scholar
- 16.A. Lateef, M. A. Azeez, T. B. Asafa, T. A. Yekeen, A. Akinboro, I. C. Oladipo, L. Azeez, S. A. Ojo, E. B. Gueguim-Kana, and L. S. Beukes (2016). Cocoa pod husk extract-mediated biosynthesis of silver nanoparticles: its antimicrobial, antioxidant and larvicidal activities. J. Nanostruct. Chem. 6, 159–169.CrossRefGoogle Scholar
- 20.R. Sriranjani, B. Srinithya, V. Vellingiri, P. Brindha, S. P. Anthony, A. Sivasubramanian, and M. S. Muthuraman (2016). Silver nanoparticle synthesis using Clerodendrum phlomidis leaf extract and preliminary investigation of its antioxidant and anticancer activities. J. Mol. Liq. 220, 926–930.CrossRefGoogle Scholar
- 26.P. C. Naha, K. C. Lau, J. C. Hsu, M. Hajfathalian, S. Mian, P. Chhour, L. Uppuluri, E. S. McDonald, A. D. Maidment, and D. P. Cormode (2016). Gold silver alloy nanoparticles (GSAN): an imaging probe for breast cancer screening with dual-energy mammography or computed tomography. Nanoscale 8, 13740–13754.CrossRefGoogle Scholar
- 27.M. K. Ballo, S. Rtimi, C. Pulgarin, N. Hopf, A. Berthet, J. Kiwi, P. Moreillon, J. M. Entenza, and A. Bizzini (2016). In vitro and in vivo effectiveness of an innovative silver–copper nanoparticle coating of catheters to prevent methicillin-resistant Staphylococcus aureus infection. Antimicrob. Agents Chemother. 60, 5349–5356.CrossRefGoogle Scholar
- 31.M. A. Azeez, A. Lateef, T. B. Asafa, T. A. Yekeen, A. Akinboro, I. C. Oladipo, E. B. Gueguim-Kana, and L. S. Beukes (2016). Biomedical applications of cocoa bean extract-mediated silver nanoparticles as antimicrobial, larvicidal and anticoagulant agents. J. Clust. Sci.. doi: 10.1007/s10876-016-1055-2.Google Scholar
- 33.A. Lateef, M. A. Akande, M. A. Azeez, S. A. Ojo, B. I. Folarin, E. B. Gueguim-Kana, and L. S. Beukes (2016). Phytosynthesis of silver nanoparticles (AgNPs) using miracle fruit plant (Synsepalum dulcificum) for antimicrobial, catalytic, anti-coagulant and thrombolytic applications. Nanotechnol. Rev. 5, 507–520.Google Scholar
- 36.S. A. Ojo, A. Lateef, M. A. Azeez, S. M. Oladejo, A. S. Akinwale, T. B. Asafa, T. A. Yekeen, A. Akinboro, I. C. Oladipo, E. B. Gueguim-Kana, and L. S. Beukes (2016). Biomedical and catalytic applications of gold and silver-gold alloy nanoparticles biosynthesized using cell-free extract of Bacillus safensis LAU 13: antifungal, dye degradation, anti-coagulant and thrombolytic activities. IEEE Trans. Nanobiosci. 15, 433–442.CrossRefGoogle Scholar
- 38.A. Lateef, M. A. Azeez, T. B. Asafa, T. A. Yekeen, A. Akinboro, I. C. Oladipo, F. E. Ajetomobi, E. B. Gueguim-Kana, and L. S. Beukes (2015). Cola nitida-mediated biogenic synthesis of silver nanoparticles using seed and seed shell extracts and evaluation of antimicrobial activities. BioNanoSci. 5, 196–205.CrossRefGoogle Scholar
- 40.A. Lateef, M. A. Azeez, T. B. Asafa, T. A. Yekeen, A. Akinboro, I. C. Oladipo, L. Azeez, S. E. Ajibade, S. A. Ojo, E. B. Gueguim-Kana, and L. S. Beukes (2016). Biogenic synthesis of silver nanoparticles using a pod extract of Cola nitida: antibacterial, antioxidant activities and application as a paint additive. J. Taibah Univ. Sci. 10, 551–562.CrossRefGoogle Scholar
- 42.C. S. Devi, V. Mohanasrinivasan, A. Tarafder, E. Shishodiya, B. Vaishnavi, and S. JemimahNaine (2016). Combination of clot buster enzymes and herbal extracts: a new alternative for thrombolytic drugs. Biocatal. Agric. Biotechnol. 8, 152–157.Google Scholar
- 43.S. Shankar, L. Jaiswal, R. S. L. Aparna, and R. G. S. V. Prasad (2014). Synthesis, characterization, in vitro biocompatibility, and antimicrobial activity of gold, silver and gold silver alloy nanoparticles prepared from Lansium domesticum fruit peel extract. Mater. Lett. 137, 75–78.CrossRefGoogle Scholar
- 45.H. Roozbahani, M. Asmar, N. Ghaemi, and K. Issazadeh (2014). Evaluation of antimicrobial activity of spider silk Pholcus phalangioides against two bacterial pathogens in food borne. Int. J. Adv. Biol. Biomed. Res. 2, 2197–2199.Google Scholar
- 48.A. C. Odebode (1996). Phenolic compounds in the kola nut (Cola nitida and Cola acuminata) (Sterculiaceae) in Africa. Rev. Biol. Trop. 44, 513–515.Google Scholar
- 49.B. B. Babatunde and R. A. Hamzat (2005). Effects of feeding graded levels of kolanut husk meal on the performance of cockerels. Niger. J. Anim. Prod. 32, 61–66.Google Scholar
- 50.E. U. Asogwa, J. C. Anikwe, and F. C. Ihokwunye (2006). Kola production and utilization for economic development. Afr. Sci. 7, 4–5.Google Scholar
- 51.C. Orwa, A. Mutua, R. Kindt, R. Jamnadass, S. Anthony, Agro-forestry tree database: a tree reference and selection guide version 4.0, 2009, http://www.worldagroforestry.org/sites/treedbs/treedatabases.asp. Accessed on 19 June 2015.
- 55.H. F. Chang and L. L. Yang (2012). Radical-scavenging and rat liver mitochondria lipid peroxidative inhibitory effects of natural flavonoids from traditional medicinal herbs. J. Med. Plants Res. 6, 997–1006.Google Scholar
- 64.World Health Organization, Global status report on non-communicable diseases, 2011, Geneva, http://www.who.int/nmh/publications/ncd_report2010/en/. Accessed on 17 October 2016.
- 65.M. J. Uddin, T. B. Emran, A. K. Nath, A. Jenny, M. Dutta, and M. M. Morshed (2013). Thrombolytic activity of Spilienthes calva and Leucas zeylanica. Mol. Clin. Pharm. 4, 32–37.Google Scholar
- 66.I. Cicha (2015). Thrombosis, novel nanomedical concepts of diagnosis and treatment. World J. Cardiol. 7, 434–441.Google Scholar