Evaluation of Some Biosynthesized Silver Nanoparticles for Biomedical Applications: Hydrogen Peroxide Scavenging, Anticoagulant and Thrombolytic Activities
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The present study examines the hydrogen peroxide scavenging, anticoagulant and thrombolytic activities of silver nanoparticles (AgNPs) that were biosynthesized using extracts obtained from spider cobweb (CB), pod (KP), seed (KS) and seed shell (KSS) of kolanut (Cola nitida). The nearly spherical shaped AgNPs, with surface plasmon resonance of 431.5–457.5 nm, were polydispersed having sizes of 3–50, 12–80, 8–50, and 5–40 nm for CB, KP, KS and KSS-AgNPs respectively. Hydrogen peroxide scavenging activities of 77–99.8% were obtained using 1–20 µg/ml of AgNPs. The particles prevented the coagulation of blood, and also showed thrombolytic activities of 55.76–89.83%, with KSS-AgNPs having the highest activity. Microscopic examination of the lyzed blood clot supported the thrombolytic activities. On the other hand, silver nitrate solution showed negligible activity of 1.92%, while thrombolysis of 7.55, 8.70, 8.93 and 30.19% were obtained for the extracts of KSS, CB, KS and KP respectively. The results herein presented showed potential biomedical applications of the biosynthesized AgNPs to scavenge free radicals and for the management of blood coagulation disorders and thrombotic diseases.
KeywordsBiosynthesis Silver nanoparticles Anticoagulant activity Thrombolytic activity Blood coagulation disorders
The provision of some facilities used in this investigation by the authority of LAUTECH, Ogbomoso, Nigeria is grateful acknowledged by A. Lateef.
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