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Early Systemic Sclerosis: Serum Profiling of Factors Involved in Endothelial, T-cell, and Fibroblast Interplay is Marked by Elevated Interleukin-33 Levels

Journal of Clinical Immunology volume 34, pages 663–668 (2014)Cite this article

Abstract

Purpose

To assess the serum profile of factors involved in endothelial, T-cell, and fibroblast interplay in patients with Raynaud’s phenomenon (RP) associated with nailfold vodeocapillaroscopy (NVC) scleroderma findings and/or systemic sclerosis (SSc) marker autoantibodies, recently labeled as early SSc patients.

Methods

Serum levels of soluble intercellular adhesion molecule-1 (sICAM-1), soluble vascular adhesion molecule-1 (sVCAM-1), CCL2, CXCL8, IL-13, IL-33, and transforming growth factor-β (TGF-β) were measured in 24 early SSc patients, 48 definite SSc patients, and 24 osteoarthritis/fibromyalgia controls by multiplex suspension immunoassay. All SSc patients were investigated for the presence/absence of preclinical and clinical organ involvement, SSc marker autoantibodies, and NVC abnormalities.

Results

Serum sICAM-1, CCL2, CXCL8, and IL-13 were increased in all SSc patients as compared to controls, and paralleled the severity of the disease subset (early SSc < limited cutaneous SSc < diffuse cutaneous SSc; p < 0.0001). Surprisingly, IL-33 was significantly higher in early SSc patients as compared to both controls (p < 0.01) and definite SSc patients (p < 0.05). In early SSc there were no differences in the investigated markers according to the functional and serological features assessed.

Conclusions

Our study suggests that an endothelial, T-cell and fibroblast activation can be present in patients with early SSc and it is associated with a distinct profile of circulating factors involved in the cross-talk of these cells. The marked increase of IL-33 in early SSc patients suggests new routes of investigation of cell-cell dynamics in target tissues predating overt disease manifestations, thus opening to new therapeutic approaches.

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Financial Support

This study was supported by a grant of the Italian Foundation for Arthritis Research (FIRA).

Conflict of Interest

All authors of this article declare they have no competing interests.

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Affiliations

  1. Rheumatology Unit, Second University of Naples, Via S. Pansini 5, 80131, Naples, Italy

    Serena Vettori, Giovanna Cuomo, Michele Iudici, Virginia D’Abrosca, Veronica Giacco & Gabriele Valentini

  2. Clinical Immunology Unit, Second University of Naples, via Pansini 5, 80131, Naples, Italy

    Giusi Barra & Raffaele De Palma

Authors
  1. Serena Vettori
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  2. Giovanna Cuomo
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  3. Michele Iudici
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  4. Virginia D’Abrosca
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  5. Veronica Giacco
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  6. Giusi Barra
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  7. Raffaele De Palma
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  8. Gabriele Valentini
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Correspondence to Serena Vettori.

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Vettori, S., Cuomo, G., Iudici, M. et al. Early Systemic Sclerosis: Serum Profiling of Factors Involved in Endothelial, T-cell, and Fibroblast Interplay is Marked by Elevated Interleukin-33 Levels. J Clin Immunol 34, 663–668 (2014). https://doi.org/10.1007/s10875-014-0037-0

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  • DOI: https://doi.org/10.1007/s10875-014-0037-0

Keywords

  • Systemic sclerosis
  • Raynaud’s
  • adhesion molecules
  • chemokines
  • cytokines
  • IL-33