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Nicotine Up-regulated 4-1BBL Expression by Activating Mek-PI3K Pathway Augments the Efficacy of Bone Marrow-Derived Dendritic Cell Vaccination

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Abstract

Purpose

To explore the role of 4-1BBL in nicotine-treated immature dendritic cells (imDCs) mediated anti-tumor effects.

Methods

Bone marrow-derived imDCs were stimulated with nicotine and 4-1BBL expression was determinated by flow cytometry, Western blot and RT-PCR respectively. Then, the roles of 4-1BBL in nicotine-augmented DCs-dependent T cell proliferation, CTL priming and anti-tumor effects were investigated by BrdU cell proliferation assay, enzyme-linked immunospot assay and in vivo preventive effect on tumor development, respectively. Finally, using relative kinase inhibitors, the mechanism of 4-1BBL up-regulation by nicotine stimulation and the roles of Mek-PI3K signal pathways in nicotine-augmented DCs-dependent T cell proliferation were explored by Western blot and BrdU cell proliferation assay, respectively.

Results

Firstly, nicotine could up-regulate 4-1BBL expression in both protein and mRNA levels. Secondly, the effects of nicotine-augmented DCs-dependent T-cell proliferation, CTL priming and anti-tumor effects could be significantly abolished by blocking CD80, CD86 and 4-1BBL activity, respectively. Thirdly, the combined blockages of CD80/CD86, CD80/4-1BBL, CD86/4-1BBL or CD80/CD86/4-1BBL signals could decrease 53.2 %, 29.6 %, 27.9 % and 54.5 % nicotine-enhanced T cell proliferation, respectively. Importantly, nicotine-induced 4-1BBL up-regulation could be decreased by the usage of Mek-PI3K pathway kinase inhibitors. The pre-treatment of Mek-p38-PI3K kinase inhibitors could obviously abolish nicotine-augmented DCs-dependent T cell proliferation.

Conclusions

CD80/CD86 and 4-1BBL are critical for nicotine augmented DCs-mediated anti-tumor effects. 4-1BBL and CD80/CD86 could be considered as potential candidates for preventive and therapeutic tumor vaccination.

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Abbreviations

imDCs:

immature dendritic cells

DCs:

Dendritic cells

nAChR:

nicotinic acetylcholine receptor

MAPK:

mitogen-activated protein kinase

PI3K:

phosphatidylinositol-3-kinase

Ni:

nicotine

MLRs:

mixed lymphocyte reactions

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Acknowledgements

The project was supported by grants from the Natural Science Foundation of Xiamen (No.3502Z20104002), Natural Science Foundation of China and the grant from the National Laboratory for Oncogenes and Related Genes of China (90-08-02). We thank Professor Cao XT (Second Military Medical University, Shanghai, China) for kindly providing EG7 cell lines and Dr. Wang N (Shanghai Cancer Institute) for polishing the manuscript. Also, we thank Jin Hua Su and Fu Chen for excellent animal care. FG. Gao designed the research and wrote the paper. HJ. Jin contributed to Western blot, MLR, ELISA and Elispot. HX. Sui contributed to DCs induction and adoptive transfer. YN. Wang contributed to flow cytometry.

Conflict of interest

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. Department of Immunology, Basic Medicine Science, Medical College, Xiamen University, Xiamen, 361005, Peoples Republic of China

    Hao Jie Jin, Hua Xiu Sui, Yi Nan Wang & Feng Guang Gao

  2. The State Key Laboratory for Oncogenes and Related Genes, Cancer Institute of Shanghai Jiao Tong University, Shanghai, 200030, Peoples Republic of China

    Feng Guang Gao

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  1. Hao Jie Jin
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  2. Hua Xiu Sui
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Correspondence to Feng Guang Gao.

Additional information

Reprints: Feng Guang Gao Department of Immunology, Basical Medicine Science, Medical College, Xiamen University, Xiamen 361005, Peoples Republic of China. Email: gfengguang@xmu.edu.cn

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Jin, H.J., Sui, H.X., Wang, Y.N. et al. Nicotine Up-regulated 4-1BBL Expression by Activating Mek-PI3K Pathway Augments the Efficacy of Bone Marrow-Derived Dendritic Cell Vaccination. J Clin Immunol 33, 246–254 (2013). https://doi.org/10.1007/s10875-012-9761-5

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  • DOI: https://doi.org/10.1007/s10875-012-9761-5

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