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Journal of Clinical Immunology

, Volume 32, Issue 6, pp 1292–1304 | Cite as

Grape Seed Proanthocyanidin Extract Attenuates Allergic Inflammation in Murine Models of Asthma

  • Taehoon Lee
  • Hyouk-Soo Kwon
  • Bo-Ram Bang
  • Yoon Su Lee
  • Mi-Young Park
  • Keun-Ai Moon
  • Tae-Bum Kim
  • Ki-Young Lee
  • Hee-Bom Moon
  • You Sook ChoEmail author
Original Research

Abstract

Background

Antioxidants have been suggested to alleviate the pathophysiological features of asthma, and grape seed proanthocyanidin extract (GSPE) has been reported to have powerful antioxidant activity.

Purpose

This study was performed to determine whether GSPE has a therapeutic effect on allergic airway inflammation in both acute and chronic murine model of asthma.

Methods

Acute asthma model was generated by intraperitoneal sensitization of ovalbumin (OVA) with alum followed by aerosolized OVA challenges, whereas chronic asthma model was induced by repeated intranasal challenges of OVA with fungal protease twice a week for 8 weeks. GSPE was administered by either intraperitoneal injection or oral gavage before OVA challenges. Airway hyperresponsiveness (AHR) was measured, and airway inflammation was evaluated by bronchoalveolar lavage (BAL) fluid analysis and histopathological examination of lung tissue. Lung tissue levels of various cytokines, chemokines, and growth factors were analyzed by quantitative polymerase chain reaction and ELISA. Glutathione assay was done to measure oxidative burden in lung tissue.

Results

Compared to untreated asthmatic mice, mice treated with GSPE showed significantly reduced AHR, decreased inflammatory cells in the BAL fluid, reduced lung inflammation, and decreased IL-4, IL-5, IL-13, and eotaxin-1 expression in both acute and chronic asthma models. Moreover, airway subepithelial fibrosis was reduced in the lung tissue of GSPE-treated chronic asthmatic mice compared to untreated asthmatic mice. Reduced to oxidized glutathione (GSH/GSSG) ratio was increased after GSPE treatment in acute asthmatic lung tissue.

Conclusion

GSPE effectively suppressed inflammation in both acute and chronic mouse models of asthma, suggesting a potential role of GSPE as a therapeutic agent for asthma.

Keywords

Proanthocyanidins grape seed extract asthma antioxidants airway remodeling 

Notes

Acknowledgments

This work was supported by a grant (No. 20090086092) from the National Research Foundation of Korea (NRF) to Y.S.C. and a grant (No.2012-302) from Asan Life and Science Institute to Y.S.C.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Taehoon Lee
    • 1
  • Hyouk-Soo Kwon
    • 1
  • Bo-Ram Bang
    • 2
  • Yoon Su Lee
    • 1
  • Mi-Young Park
    • 2
  • Keun-Ai Moon
    • 2
  • Tae-Bum Kim
    • 1
  • Ki-Young Lee
    • 3
  • Hee-Bom Moon
    • 1
  • You Sook Cho
    • 1
    Email author
  1. 1.Department of Allergy and Clinical Immunology, Asan Medical CenterUniversity of Ulsan College of MedicineSeoulKorea
  2. 2.Asan Institute for Life ScienceSeoulKorea
  3. 3.Department of Molecular Cell BiologySungkyunkwan University School of MedicineSuwonKorea

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