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Cognate Th2–B Cell Interaction is Essential for the Autoantibody Production in Pemphigus Vulgaris

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Abstract

Pemphigus vulgaris (PV) is a Th2-dominant autoimmune skin disease. We showed that indeed active PV patients had a biased Th2 response and specific IgG4 autoantibodies were dominant. To further investigate the role of antigen-specific Th2 cells in the regulation of pathogenic Dsg3-IgG antibody production, we used recombined Dsg3 protein to immunize wild-type C57BL/6 mice with aluminum hydroxide or complete Freund’s adjuvant as adjuvant. CD4+ T cells from Dsg3-immunized mice were adoptively transferred into TCR-β chain deficient mice. The transferred CD4+ T cells were readily seen in the peripheral blood and spleen, and interacted with B cells, resulting in B-cell activation. Furthermore, transferred CD4+ T cells from mice immunized with Dsg3 plus Alum with Th2 phenotype were able to render unprimed B cells to secrete Dsg3-specific IgG1 antibody in vivo. Taken together, these results provide the first demonstration of direct role of Dsg3-reactive CD4+ T (Th2) cells in the regulation of pathologic anti-Dsg3 antibody production.

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (No. 30771932, 81171499). We thank the staff of Shanghai Institute of Immunology, Institute of Medical Science, School of Medicine, Shanghai Jiao Tong University, for their technical assistance.

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Authors and Affiliations

  1. Department of Dermatology, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, 197 Rui Jin Er Road, Shanghai, China

    Haiqin Zhu, Yayuan Chen, Jie Zheng & Meng Pan

  2. Shanghai Institute of Immunology, School of Medicine, Shanghai Jiaotong University, Shanghai, China

    Yun Zhou & Ying Wang

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  1. Haiqin Zhu
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  2. Yayuan Chen
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  3. Yun Zhou
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  4. Ying Wang
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  5. Jie Zheng
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  6. Meng Pan
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Correspondence to Meng Pan.

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Zhu, H., Chen, Y., Zhou, Y. et al. Cognate Th2–B Cell Interaction is Essential for the Autoantibody Production in Pemphigus Vulgaris. J Clin Immunol 32, 114–123 (2012). https://doi.org/10.1007/s10875-011-9597-4

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  • DOI: https://doi.org/10.1007/s10875-011-9597-4

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