Journal of Clinical Immunology

, Volume 31, Issue 6, pp 1054–1064 | Cite as

Kinetics of Effector Functions and Phenotype of Virus-Specific and γδ T Lymphocytes in Primary Human Cytomegalovirus Infection During Pregnancy

  • Chiara Fornara
  • Daniele Lilleri
  • M. Grazia Revello
  • Milena Furione
  • Maurizio Zavattoni
  • Elisa Lenta
  • Giuseppe Gerna
Article

Abstract

The T-cell response to human cytomegalovirus (HCMV) primary infection was analyzed in 27 pregnant women during the first year after primary HCMV infection. Pregnant women with remote HCMV infection were enrolled as controls. Interferon-γ-producing T cells were readily detected at levels comparable (CD4+) or higher (CD8+) than controls, whereas the CD4+ and CD8+ lymphoproliferative response as well as IL-2 production was significantly reduced with respect to controls for at least 9 months after infection. In addition, CD45RA re-expression as well as cytotoxic T lymphocyte activity and perforin expression were the major components of the adaptive CD4+ and CD8+ T-cell immune response, while Vδ2 γδ T-cell expansion in response to HCMV infection followed kinetics similar to that of CD8+ T cells. Reduced CD45RA re-expression directly correlated with HCMV transmission to the fetus, thus providing an important prognostic parameter.

Keywords

T-cell immune response γδ T cells primary HCMV infection cytotoxicity perforin 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Chiara Fornara
    • 1
    • 2
  • Daniele Lilleri
    • 1
  • M. Grazia Revello
    • 3
  • Milena Furione
    • 4
  • Maurizio Zavattoni
    • 4
  • Elisa Lenta
    • 4
  • Giuseppe Gerna
    • 1
  1. 1.Experimental Research Laboratories, Transplantation AreaFondazione IRCCS Policlinico San MatteoPaviaItaly
  2. 2.Experimental Research Laboratories, Biotechnology AreaFondazione IRCCS Policlinico San MatteoPaviaItaly
  3. 3.Department of Obstetrics and GynaecologyFondazione IRCCS Policlinico San MatteoPaviaItaly
  4. 4.Molecular Virology Unit, Virology and Microbiology ServiceFondazione IRCCS Policlinico San MatteoPaviaItaly

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