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Elevated Th22 Cells Correlated with Th17 Cells in Patients with Rheumatoid Arthritis

Journal of Clinical Immunology volume 31, pages 606–614 (2011)Cite this article

Abstract

Background

T-helper (Th) 22 and Th17 cells are implicated in the pathogenesis of autoimmune diseases. The roles of Th22 cells in the pathophysiology of rheumatoid arthritis (RA) remain unsettled.

Materials and Methods

CD4+IFNγ−IL17−IL-22+ T cells (Th22 cells), CD4+IFNγ−IL-22−IL17+ T cells (pure Th17 cells), CD4+IL17+ T cells (Th17 cells), and CD4+IFNγ+ T cells (Th1 cells) in RA, osteoarthritis patients, and healthy controls were examined by flow cytometry. Plasma IL-22 and IL-17 levels were examined by enzyme-linked immunosorbent assay.

Results

Th22 cells, pure Th17 cells, Th17 cells, and interleukin-22 were significantly elevated in RA patients compared with osteoarthritis and healthy controls, but there were no significant differences regarding Th1 cells and interleukin-17. Th22 cells showed a positive correlation with interleukin-22 as well as pure Th17 cells or Th17 cells in RA patients. Additionally, the percentages of Th22 cells, pure Th17 cells as well as Th17 cells correlated positively with both C-reactive protein levels and 28-joints disease activity score.

Conclusion

Together, our results indicated a possible role of Th22 pure Th17 cells and Th17 cells in RA, and blockade of the interleukin-22 may be a reasonable therapeutic strategy for RA.

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Abbreviations

RA:

Rheumatoid arthritis

OA:

Osteoarthritis

CRP:

C-reactive protein

DAS28:

28-joints disease activity score

PB:

Peripheral blood

SF:

Synovial fluid

SLE:

Systemic lupus erythematosus

SS:

Systemic sclerosis

IBD:

Inflammatory bowel disease

PDGF:

Platelet-derived growth factor

CCR:

Chemokine receptor

ERK1/2:

Extracellular regulated kinase1/2

MAPK:

Mitogen-activated protein kinases

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Acknowledgments

This study was partially supported by research funding from the National Natural Science Foundation (30600680, 81070407, and 30973018), the Shandong Technological Development Project (2005BS03022, Q2008C07, and BS2009SW014), and the SRF for ROCS, SEM, GIIFSDU (yzc10147), and IIFSDU (2009TS063, 2009TS071).

Disclosures

The authors have no financial conflict of interest.

Author information

Affiliations

  1. Department of Orthopedics, Qilu Hospital, Shandong University, Jinan, 250012, China

    Lei Zhang, Jian-min Li & Yong-gang Li

  2. Department of Hematology, Qilu Hospital, Shandong University, 107 West Wenhua Road, Jinan, Shandong, 250012, China

    Xin-guang Liu, Dao-xin Ma, Yu Hu & Shuang Yu

  3. Department of Rheumatology, Provincial Hospital affiliated to Shandong University, Jinan, 250021, China

    Nai-wen Hu

  4. Department of Clinical Laboratory, Qilu Hospital, Shandong University, Jinan, 250012, China

    Wei Li & Guang-hui Wang

  5. Institute of Basic Medical Sciences, Qilu Hospital, Shandong University, Jinan, 250012, China

    Xun Qu, Mei-xiang Yang & A-lei Feng

Authors
  1. Lei Zhang
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  2. Jian-min Li
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  3. Xin-guang Liu
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  7. Wei Li
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  8. Yu Hu
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  10. Xun Qu
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  13. Guang-hui Wang
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Corresponding author

Correspondence to Dao-xin Ma.

Additional information

Lei Zhang, Jian-min Li, and Xin-guang Liu contributed equally to this study.

Electronic Supplementary Material

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Supplementary Table 1

Frequencies of T-helper cells and the related ratio in RA, OA patients, and healthy controls (DOC 30 kb)

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Zhang, L., Li, Jm., Liu, Xg. et al. Elevated Th22 Cells Correlated with Th17 Cells in Patients with Rheumatoid Arthritis. J Clin Immunol 31, 606–614 (2011). https://doi.org/10.1007/s10875-011-9540-8

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  • DOI: https://doi.org/10.1007/s10875-011-9540-8

Keywords

  • Rheumatoid arthritis
  • T helper 22
  • T helper 17
  • T helper 1
  • Interleukin-22
  • Interleukin-17