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CXCL10 and CXCL13 Expression were Highly Up-regulated in Peripheral Blood Mononuclear Cells in Acute Rejection and Poor Response to Anti-Rejection Therapy

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Abstract

Background

Acute rejection is still one of the main complications which enhances the cost and the risk to renal graft failure. Chemokines, interacting with respective receptors, can recruit leukocytes into grafts and mediate allograft rejection. In this study, we aimed to analyze gene expression of chemokines including CCL5/RANTES, CXCL10/IP-10, CXCL13/BCA-1, and receptors of CCR5, CXCR3, CXCR5 in peripheral blood mononuclear cells (PBMCs) during acute renal allograft rejection

Methods

Gene expression of all these chemokines and receptors in PBMCs were analyzed by real-time PCR from 14 stable recipients, 32 biopsy-proven acute rejection (AR), and 5 acute tubular necrosis (ATN).

Results

Gene expression of CCL5, CXCL10, CXCL13, and CCR5 were up-regulated both in AR and ATN group compared to stable recipients (fold change > 2, P < 0.05). Serum creatinine recovered to baseline level after anti-rejection therapy was defined as AR-sensitive and creatinine maintained above 200 μmol/L as AR-resistant. Expression of CXCL10 and CXCL13 were 5.98-, 2.94-, and 20.5, 10.8-fold change in AR-resistant and AR-sensitive compared to stable recipients, respectively. The expression of CXCL10 and CXCL13 was a twofold change in AR-resistant compared to AR-sensitive recipients (P < 0.05). Five out of ten AR-resistant recipients lost graft function in the follow-up.

Conclusion

CXCL10 and CXCL13 expression were highly up-regulated in PBMCs in acute renal allograft rejection, especially in poor response to anti-rejection therapy and detrimental prognosis.

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Acknowledgments

This study was supported by Key Projects in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period (2008BAI60B04) and a major project of Zhejiang National Science & Technology bureau (2008C13026-2) and the National High Technology Research and Development Program of China (863 Program, 2006AA02A248).

Conflict of interest

None declared.

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Authors and Affiliations

  1. Kidney Disease Center, the First Affiliated Hospital of Medical College, Zhejiang University, 79 Qingchun road, Hangzhou, 310003, China

    Youying Mao, Minmin Wang, Qin Zhou, Juan Jin, Yucheng Wang, Wenhan Peng, Jianyong Wu, Zhangfei Shou & Jianghua Chen

  2. Key Lab of Combined Multi-Organ Transplantation, Ministry of Public Health, the First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou, China

    Jianyong Wu, Zhangfei Shou & Jianghua Chen

  3. Kidney Disease Immunology Laboratory, The Third Grade Laboratory, State Administration of Traditional Chinese Medicine, Hangzhou, China

    Youying Mao, Minmin Wang, Qin Zhou, Juan Jin, Yucheng Wang, Jianyong Wu, Zhangfei Shou & Jianghua Chen

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  1. Youying Mao
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  2. Minmin Wang
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  3. Qin Zhou
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  4. Juan Jin
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  5. Yucheng Wang
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  6. Wenhan Peng
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  7. Jianyong Wu
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  8. Zhangfei Shou
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  9. Jianghua Chen
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Corresponding author

Correspondence to Jianghua Chen.

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Mao, Y., Wang, M., Zhou, Q. et al. CXCL10 and CXCL13 Expression were Highly Up-regulated in Peripheral Blood Mononuclear Cells in Acute Rejection and Poor Response to Anti-Rejection Therapy. J Clin Immunol 31, 414–418 (2011). https://doi.org/10.1007/s10875-010-9500-8

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  • DOI: https://doi.org/10.1007/s10875-010-9500-8

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