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Association and Expression Study of PRKCH Gene in a French Caucasian Population with Rheumatoid Arthritis

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Abstract

We study the association between three protein kinase C, eta gene polymorphisms (+8134C/T, rs912620, rs959728), and susceptibility to rheumatoid arthritis. One hundred French Caucasian rheumatoid arthritis trio families were genotyped. Relative quantification of protein kinase C, eta mRNA expression was performed from whole blood in 24 unrelated rheumatoid arthritis patients and in 16 healthy controls. Our results showed no significant association or linkage between the protein kinase C, eta polymorphisms, and rheumatoid arthritis. The protein kinase C, eta mRNA was expressed at lower level in rheumatoid arthritis unrelated patients than in healthy controls. This study shows that protein kinase C, eta gene is not a Rheumatoid Arthritis major susceptibility genetic factor in the French Caucasian population. Furthermore, the lower expression of this gene in rheumatoid arthritis patients comparing to healthy controls suggests that protein kinase C, eta could be associated with the patho-physiologic mechanism of rheumatoid arthritis.

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Acknowledgments

This work was supported by AFSSAPS, Association Française des Polyarthritiques, Société Française de Rhumatologie, Association Rhumatisme et Travail, Association Polyarctique, Groupe Taitbout, Genopole®, Conseil Régional Ile de France, Fondation pour la Recherche Médicale, Université Evry-Val d’Essonne and unrestricted institutional support from Wyeth, Schering-Plough, Pfizer, and Amgen. Vitor Hugo Teixeira’s work was supported by Foundation for Science and Technology, Portugal (grant SFRH/BD/23304/2005). We are grateful to the RA patients, their families, and rheumatologists for participation in this study. We thank Dr Robert Olaso (Centre National de Génotypage, France), Fréderique Bitton, Samuel Mainguet, Dr Fabiana Heredia, and Filipe Teixeira (French National Institute for Agricultural Research, France).

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Authors and Affiliations

  1. GenHotel-EA3886, Evry-Paris VII Universities, Evry-Genopole, France

    Vitor Hugo Teixeira, Laurent Jacq, Jeoiakim Moore, Sandra Lasbleiz, François Cornelis & Elisabeth Petit-Teixeira

  2. Faculty of Medicine, University of Coimbra, Coimbra, Portugal

    Vitor Hugo Teixeira

  3. Centre Hospitalier Sud Francilien, Corbeil-Essonnes, France

    Laurent Jacq, Pascal Hilliquin & François Cornelis

  4. Service de Rhumatologie, Hôpital Lariboisière, Paris, France

    Sandra Lasbleiz

  5. Center for Neurosciences and Cell Biology, Faculty of Medicine, University of Coimbra, Coimbra, Portugal

    Catarina Resende Oliveira

  6. Unité de Génétique Clinique, Hôpital Lariboisière, Paris, France

    François Cornelis

  7. Genhotel-EA3886, Laboratoire Européen de Recherche pour la Polyarthrite Rhumatoïde, 2 Rue Gaston Crémieux, 91057, Evry-Genopole Cedex, France

    Vitor Hugo Teixeira

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  1. Vitor Hugo Teixeira
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  2. Laurent Jacq
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  3. Jeoiakim Moore
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  4. Sandra Lasbleiz
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  5. Pascal Hilliquin
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  6. Catarina Resende Oliveira
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  7. François Cornelis
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  8. Elisabeth Petit-Teixeira
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Correspondence to Vitor Hugo Teixeira.

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Teixeira, V.H., Jacq, L., Moore, J. et al. Association and Expression Study of PRKCH Gene in a French Caucasian Population with Rheumatoid Arthritis. J Clin Immunol 28, 115–121 (2008). https://doi.org/10.1007/s10875-007-9143-6

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  • DOI: https://doi.org/10.1007/s10875-007-9143-6

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