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Inhibition of Airway Allergic Disease by Co-Administration of Flagellin with Allergen

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Abstract

Bacterial flagellin, which activates Toll-like receptor 5 and cytosolic pattern recognition receptor Ipaf, has a strong immunomodulatory activity. In the present study, we examined whether intranasal co-administration of flagellin with allergen could modulate established airway hyperresponsiveness and Th2 response using an ovalbumin (OVA)-sensitized mouse model. Balb/c mice sensitized with OVA were treated with OVA–flagellin (FlaB) mixture three times at 1-week intervals. Seven days after the final OVA–FlaB administration, the mice were challenged with OVA inhalation, and airway responses and OVA-specific immune responses were evaluated. The OVA–FlaB treatment significantly suppressed OVA-induced airway hyperresponsiveness, airway eosinophilic inflammation, and OVA-specific Th2 cytokine productions in splenocytes. These results indicate that flagellin co-administered with allergen can modulate airway inflammatory response through inhibition of Th2 responses, and flagellin can be considered as a component for allergen-specific immunotherapy.

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Acknowledgment

We would like to thank Yong-A Koh for the excellent technical assistance. This work was supported by grant from the Regional Technology Innovation Program of the Ministry of Commerce, Industry and Energy, Republic of Korea (RTI05-01-01) and from Korea Health 21R&D project by the Ministry of Health and Welfare, Republic of Korea (A010003).

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Authors and Affiliations

  1. Clinical Vaccine R&D Center, Chonnam National University, Gwangju, South Korea

    Shee Eun Lee, Young Ran Kim, Soo Young Kim, Soo Jang Bae, Young Jong Ko, Hwa-Ja Ryu, Hyon E. Choy & Joon Haeng Rhee

  2. Genome Research Center for Enteropathogenic Bacteria, Chonnam National University, Gwangju, South Korea

    Shee Eun Lee, Soo Young Kim, Soo Jang Bae, Young Jong Ko, Hwa-Ja Ryu, Hyon E. Choy & Joon Haeng Rhee

  3. Chonnam Dental Research Institute and The 2nd Stage of Brain Korea 21 for School of Dentistry, Chonnam National University, Gwangju, South Korea

    Shee Eun Lee, Mi-Kwang Kim, Hwa-Ja Ryu & Jeong Tae Koh

  4. Department of Microbiology, Chonnam National University Medical School, Gwangju, 501-746, South Korea

    Soo Young Kim

  5. Department of Allergy, Chonnam National University Medical School, Gwangju, 501–746, South Korea

    Youngil I. Koh

  6. Department of Pathology, Chonnam National University Medical School, Gwangju, South Korea

    Jong Hee Nam

  7. Department of Oriental Medicine Materials, Dongshin University, 520–724, Naju, Chonnam, South Korea

    Young Ran Kim

  8. National Institute of Scientific Investigation, Chonnam, South Korea

    Yoo Duk Choi

  9. Clinical Vaccine R&D Center and Chonnam Dental Research Institute, Chonnam National University, 300 Yongbong-Dong, Puk-Ku, Gwangju, 500-757, South Korea

    Shee Eun Lee

  10. Clinical Vaccine R&D Center and Department of Microbiology, Chonnam National University Medical School, 5 Hak-Dong, Dong-Ku, Gwangju, 501-746, South Korea

    Joon Haeng Rhee

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  1. Shee Eun Lee
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  2. Youngil I. Koh
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Correspondence to Shee Eun Lee or Joon Haeng Rhee.

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Lee, S.E., Koh, Y.I., Kim, MK. et al. Inhibition of Airway Allergic Disease by Co-Administration of Flagellin with Allergen. J Clin Immunol 28, 157–165 (2008). https://doi.org/10.1007/s10875-007-9138-3

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  • DOI: https://doi.org/10.1007/s10875-007-9138-3

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