Fetal Cord Blood: Aspects of Heightened Immune Responses
Neonatal immune responses have been associated with the development of atopy in childhood. We assessed in cord blood mononuclear cells (CBMC) whether increased allergen/mitogen-induced lymphoproliferation (LP) is associated with pro-allergic Th2 cytokine IL-13 or Th1 cytokine IFN-γ secretion. We determined whether LP to one allergen is related to heightened lymphocyte function to other allergens/mitogen. CBMC from 135 neonates were stimulated with house dust mite (Derf1), cockroach, ovalbumin, or mitogen. LP to one allergen was associated with significantly increased LP to other allergens/mitogen. Increased Derf1-LP was associated with increased Derf1-induced IL-13 secretion (r = 0.21, p = 0.01). After adjusting for neonatal gender, race, and maternal smoking, Derf1-LP remained associated with Derf1-IL-13 (OR 3.08, 95% CI 1.56–6.10). Increased mitogen-induced proliferation was associated with increased mitogen-induced IL-13 secretion (r = 0.37, p < 0.001). For some individuals, a predisposition to a heightened immune response is already evident at birth. Whether this phenotype results in atopy in childhood warrants further investigation.
Key WordsAllergens cord blood immunology lymphocytes neonatal
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- 1.Mannino DM, Homa DM, Akinbami LJ, Moorman JE, Gwynn C, Redd SC: Surveillance for asthma—United States, 1980–1999. MMWR Surveill Summ 51:1, 2002Google Scholar
- 7.Kondo N, Kobayashi Y, Shinoda S, Takenaka R, Teramoto T, Kaneko H, Fukao T, Matsui E, Kasahara K, Yokoyama Y: Reduced interferon gamma production by antigen-stimulated cord blood mononuclear cells is a risk factor of allergic disorders—6-year follow-up study. Clin Exp Allergy 28:1340, 1998CrossRefPubMedGoogle Scholar
- 21.Upham JW, Holt BJ, Baron-Hay MJ, Yabuhara A, Hales BJ, Thomas WR, Loh RK, O’Keeffe PT, Palmer L, Le Souef PN, et al.: Inhalant allergen-specific T-cell reactivity is detectable in close to 100% of atopic and normal individuals: Covert responses are unmasked by serum-free medium. Clin Exp Allergy 25:634, 1995PubMedGoogle Scholar