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Probing the binding of morin with alpha-2-macroglobulin using multi-spectroscopic and molecular docking approach

Interaction of morin with α2M

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Abstract

Alpha-2-macroglobulin (α2M) is an essential antiproteinase that is widely distributed in human plasma. The present study was aimed at investigating the binding of a potential therapeutic dietary flavonol, morin, with human α2M using a multi-spectroscopic and molecular docking approach. Recently, flavonoid-protein interaction has gained significant attention, because a majority of dietary bioactive components interact with proteins, thereby altering their structure and function. The results of the activity assay exhibited a 48% reduction in the antiproteolytic potential of α2M upon interaction with morin. Fluorescence quenching tests unequivocally confirmed quenching in the fluorescence of α2M in the presence of morin, conforming complex formation and demonstrating that the binding mechanism involves a dynamic mode of interaction. Synchronous fluorescence spectra of α2M with morin showed perturbation in the microenvironment around tryptophan residues. Furthermore, structural changes were observed through CD and FT-IR, showing alterations in the secondary structure of α2M induced by morin. FRET further supports the results of the dynamic mode of quenching. Moderate interaction is shown by binding constant values using Stern–Volmer’s fluorescence spectroscopy. Morin binds to α2M at 298 K with a binding constant of 2.7 × 104 M−1, indicating the strength of the association. The α2M-morin system was found to have negative ΔG values, which suggests that the binding process was spontaneous. Molecular docking also reveals the different amino acid residues involved in this binding process, revealing that the binding energy is -8.1 kcal/mol.

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Data availability

The data generated in the study would be available on reasonable request from the corresponding author.

Abbreviations

α2M:

Alpha-2-macroglobulin

PDB:

Protein data bank

FRET:

Föster resonance energy transfer

CD:

Circular dichroism

FTIR:

Förster transform infrared

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Acknowledgements

The authors wish to thank the Department of Science and Technology and the University Grants Commission (UGC), Government of India for the departmental financial support. MKZ acknowledges ICMR for providing Research Associate Fellowship.

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Facilities provided by the Department of Biochemistry, Aligarh Muslim University, Aligarh are gratefully acknowledged.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, 202002, India

    Sana Ansari, Mohammad Khalid Zia, Shamila Fatima & Fahim H. Khan

  2. Department of Biochemistry, Faculty of Dentistry, Jamia Millia Islamia, New Delhi, 110025, India

    Haseeb Ahsan

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  1. Sana Ansari
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Contributions

SA: methodology and validation; investigation and data collection; data analysis and interpretation; writing—original draft preparation. MKZ: data analysis and interpretation; supervision; funding acquisition. SF: formal analysis; data analysis and interpretation. HA: resources; writing—original draft preparation; writing—review and editing; formal analysis. FHK: conceptualization and design; supervision; project administration; funding acquisition.

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Correspondence to Fahim H. Khan.

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Ansari, S., Zia, M.K., Fatima, S. et al. Probing the binding of morin with alpha-2-macroglobulin using multi-spectroscopic and molecular docking approach. J Biol Phys 49, 235–255 (2023). https://doi.org/10.1007/s10867-023-09629-z

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