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Photoacoustic spectroscopy applied to the direct detection of bioactive compounds in Agaricus brasiliensis mycelium

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Abstract

This paper describes the application of the photoacoustic spectroscopic (PAS) for detection of bioactive compounds in Agaricus brasiliensis mycelium. The mycelium was cultivated by solid-state fermentation and by submerged fermentation. Vegetal residues from food industry were used as substrates for fermentation: apple pomace (Malus domestica), wheat (Triticum aestivum), peel and pomace of pineapple (Ananas comosus), malt (Hordeum vulgare) and grape pomace (Vitis vinifera). Dry and ground samples of biomass were directly put into the PA cell. The optical absorption spectra indicated the existence of three main absorption bands: one around 280 nm related to phytosterols (ergosterol), phenolic acids, flavonoids and aromatic amino acids, another at 340 nm, due to phenolic and flavonoid compounds, and the third one at around 550 nm associated with anthocyanins and anthocyanidins. A correlation between the PA signal and the total phenolic content was satisfactory, as well as for the analyzed spectrum region (270 nm up to 1000 nm), using multivariate methods. Our results indicated that PA technique may be considered as an analytical tool to quickly detect bioactive compounds in mushrooms without the need of sample pretreatment.

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Acknowledgements

The authors acknowledge the support from the Brazilian agencies CAPES, CNPq, SETI and Fundação Araucária.

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Authors and Affiliations

  1. Department of Physics, State University of Midwest (UNICENTRO), 85040-080, Guarapuava, Paraná, Brazil

    Fernando Maia de Oliveira & Pedro Pablo González-Borrero

  2. Department of Chemistry, State University of Midwest (UNICENTRO), 85040-080, Guarapuava, Paraná, Brazil

    João Benhur Mokochinski & Yohandra Reyes Torres

  3. Department of Food Engineering, State University of Midwest (UNICENTRO), 85040-080, Guarapuava, Paraná, Brazil

    Herta Stutz Dalla Santa

Authors
  1. Fernando Maia de Oliveira
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  2. João Benhur Mokochinski
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  3. Yohandra Reyes Torres
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  4. Herta Stutz Dalla Santa
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  5. Pedro Pablo González-Borrero
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Correspondence to Pedro Pablo González-Borrero.

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Fernando Maia de Oliveira declares that he has no conflict of interest. João Benhur Mokochinski declares that he has no conflict of interest. Yohandra Reyes Torres declares that she has no conflict of interest. Herta Stutz Dalla Santa declares that she has no conflict of interest. Pedro Pablo González Borrero declares that he has no conflict of interest.

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de Oliveira, F.M., Mokochinski, J.B., Reyes Torres, Y. et al. Photoacoustic spectroscopy applied to the direct detection of bioactive compounds in Agaricus brasiliensis mycelium. J Biol Phys 44, 93–100 (2018). https://doi.org/10.1007/s10867-017-9478-z

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  • DOI: https://doi.org/10.1007/s10867-017-9478-z

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