Journal of Biological Physics

, Volume 43, Issue 3, pp 367–379 | Cite as

The effect of low- and high-power microwave irradiation on in vitro grown Sequoia plants and their recovery after cryostorage

  • A. Halmagyi
  • E. Surducan
  • V. Surducan
Original Paper


Two distinct microwave power levels and techniques have been studied in two cases: low-power microwave (LPM) irradiation on in vitro Sequoia plants and high-power microwave (HPM) exposure on recovery rates of cryostored (−196°C) Sequoia shoot apices. Experimental variants for LPM exposure included: (a) in vitro plants grown in regular conditions (at 24 ± 1°C during a 16-h light photoperiod with a light intensity of 39.06 μEm−2 s−1 photosynthetically active radiation), (b) in vitro plants grown in the anechoic chamber with controlled environment without microwave irradiation, and (c) in vitro plants grown in the anechoic chamber with LPM irradiation for various times (5, 15, 30, 40 days). In comparison to control plants, significant differences in shoot multiplication and growth parameters (length of shoots and roots) were observed after 40 days of LPM exposure. An opposite effect was achieved regarding the content of total soluble proteins, which decreased with increasing exposure time to LPM. HPM irradiation was tested as a novel rewarming method following storage in liquid nitrogen. To our knowledge, this is the first report using this type of rewarming method. Although, shoot tips subjected to HPM exposure showed 28% recovery following cryostorage compared to 44% for shoot tips rewarmed in liquid medium at 22 ± 1 °C, we consider that the method represent a basis and can be further improved. The results lead to the overall conclusion that LPM had a stimulating effect on growth and multiplication of in vitro Sequoia plants, while the HPM used for rewarming of cryopreserved apices was not effective to achieve high rates of regrowth after liquid nitrogen exposure.


Cryopreservation Sequoia Irradiation Microwaves Redwood 



Financial support from the National Authority for Scientific Research and Innovation - ANCSI, Core Programme, PN16-30 01 02 (Nucleu-INCDTIM Cluj-Napoca, Romania) and the national program PN16 19 Biodivers (NIRDBS Romania) is gratefully acknowledged.

Author contributions

A.H., E.S., and V.S. planned and conducted the experiments and contributed to the writing of manuscript. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.


  1. 1.
    World Health Organization: Electromagnetic fields and public health: mobile phones, Fact sheet N°193 (2011)Google Scholar
  2. 2.
    Brantner, A., Lücke, W.: Influence of physical parameters on the germ-reducing effect of microwave irradiation on medicinal plants. Pharmazie 50, 762–766 (1995)Google Scholar
  3. 3.
    Shimomachi, T., Takemasa, T., Takakura, T., Kurata, K.: Nondestructive detection of plant water stress by microwave sensing. Acta Hortic. 710, 465–470 (2006)CrossRefGoogle Scholar
  4. 4.
    Soran, M.L., Stan, M., Niinemets, Ü., Copolovici, L.: Influence of microwave frequency electromagnetic radiation on terpene emission and content in aromatic plants. J Plant Physiol. 171, 1436–1443 (2014)CrossRefGoogle Scholar
  5. 5.
    Balint, V.C., Surducan, V., Surducan, E., Oroian, I.G.: Plant irradiation device in microwave field with controlled environment. Comput Electron Agric. 121, 48–56 (2016)CrossRefGoogle Scholar
  6. 6.
    Ragha, L., Mishra, S., Ramachandran, V., Bhatia, M.S.: Effects of low-power microwave fields on seed germination and growth rate. J Electromagn Anal Appl. 3, 165–171 (2011)Google Scholar
  7. 7.
    Shimomachi, T., Takemasa, T., Kurata, K., Kobashigawa, C., Omoda, E., Takakura, T.: Quantitave estimation method of plant adaptation responses to saline environment using microwave sensing. Acta Hortic. 797, 463–468 (2008)Google Scholar
  8. 8.
    Fuangfoong, M., Eaipresertsak, K., Chim-Oye, T., Dugkanya, K.: Effect of low-power microwave radiation on seed growth rate, pp. 676–679. PIERS Proceedings, Taipei (2013)Google Scholar
  9. 9.
    Vian, A., Davies, E., Gendraud, M., Bonnet, P.: Plant responses to high frequency electromagnetic fields. BioMed Res Intl. 2016, pp. 13 (2016)Google Scholar
  10. 10.
    Buchachenko, A.: Why magnetic and electromagnetic effects in biology are irreproducible and contradictory. Bioelectromagnetics 37, 1–13 (2016)Google Scholar
  11. 11.
    Surducan, E., Surducan, V., Halmagyi, A.: Process and installation for stimulating plant growth in microwave field. Romanian patent RO 125068 B1 (2012)Google Scholar
  12. 12.
    Lambardi, M.: Cryopreservation of germplasm of Populus (Poplar) species. In: Towill, L.E., Bajaj, Y.P.S. (eds.) Biotechnology in Agriculture and Forestry 50, pp. 269–286. Springer, Berlin (2002)Google Scholar
  13. 13.
    Nordin, M.S., Saad, M.S.: Genetic considerations in field genebank conservation. In: Saad, M.S., Rao, V.R. (eds.) Establishment and Management of Field Genebank, pp. 66–72. International Plant Genetic Resources Institute, Serdang (2001)Google Scholar
  14. 14.
    Pâques, M., Monod, V., Poissonnier, M., Dereuddre, J.: cryopreservation of Eucalyptus sp. shoot tips by the encapsulation-dehydration procedure. In: Towill, L.E., Bajaj, Y.P.S. (eds.) Biotechnology in Agriculture and Forestry 50, pp. 234–245. Springer, Berlin (2002)Google Scholar
  15. 15.
    IUCN: IUCN Red List of Threatened Species. (2007)
  16. 16.
    Noss, R.F.: The Redwood Forest: History, Ecology and Conservation of the Coast Redwood. Island Press, Washington DC (2000)Google Scholar
  17. 17.
    ICNIRP: Guidelines for limiting exposure to time-varying electric, magnetic and electromagnetic fields (up to 300 GHz). Health Phys. 74, 494–522 (1998)Google Scholar
  18. 18.
    Hore, P.J.: Are biochemical reactions affected by weak magnetic field? Proc. Natl. Acad. Sci. U.S.A. 109, 1357–1358 (2012)Google Scholar
  19. 19.
    Vian, A., Roux, D., Girard, S., Bonnet, P., Paladian, F., Davies, E., Ledoigt, G.: Microwave irradiation affects gene expression in plants. Plant Signal Behav. 1, 67–70 (2006)CrossRefGoogle Scholar
  20. 20.
    Surducan, E., Surducan, V.: Process and installation for dynamic substance processing in power microwave field. Romanian patent RO 122063 B1 (2008)Google Scholar
  21. 21.
    Surducan, E., Surducan, V., Keul, A., Halmagyi, A.: Microwaves irradiation experiments on biological samples. Studia UBB Biologia. LVIII, 83–98 (2013)Google Scholar
  22. 22.
    Surducan, E., Iancu, D., Glossner, J.: Microstrip multi-band composite antenna. International patent (WIPO) WO 2006086194 A2 (2006)Google Scholar
  23. 23.
    Surducan, E., Surducan, V.: Method and transducer for measuring the temperature in the microwave power processing treatments. Romanian patent RO 125999 A2 (2009)Google Scholar
  24. 24.
    Surducan, E., Surducan, V.: Thermographic transducer for microwave radiation. Romanian patent, RO 116506 B1 (2001)Google Scholar
  25. 25.
    Murashige, T., Skoog, F.A.: Revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 15, 473–497 (1962)CrossRefGoogle Scholar
  26. 26.
    Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principles of protein-dye binding. Anal Biochem. 72, 248–254 (1976)CrossRefGoogle Scholar
  27. 27.
    Halmagyi, A., Deliu, C.: Cryopreservation of redwood (Sequoia sempervirens (D. Don.) Endl.) shoot apices by encapsulation-dehydration. Contrib Bot. 46, 117–125 (2011)Google Scholar
  28. 28.
    Martínez, M.T., Ballester, A., Vieitez, A.M.: Cryopreservation of embryogenic cultures of Quercus robur using desiccation and vitrification procedures. Cryobiology 46, 182–189 (2003)Google Scholar
  29. 29.
    Halgamuge, M.N., Yak, S.K., Eberhardt, J.L.: Reduced growth of soybean seedlings after exposure to weak microwave radiation from GSM 900 mobile phone and base station. Bioelectromagnetics 36, 87–95 (2015)Google Scholar
  30. 30.
    Haneda, T., Fujimura, Y., Iino, M.: Magnetic field exposure stiffens regenerating plant protoplast cell walls. Bioelectromagnetics 27, 98–104 (2006)Google Scholar
  31. 31.
    Akbal, A., Kiran, Y., Sahin, A., Turgut-Balik, D., Balik, H.H.: Effects of electromagnetic waves emitted by mobile phones on germination, root growth, and root tip cell mitotic division of Lens culinaris Medik. Pol J Environ Stud. 21, 23–29 (2012)Google Scholar
  32. 32.
    Chen, R.D., Tabaeizadeh, Z.: Alteration of gene expression in tomato plants (Lycopersicon esculentum) by drought and salt stress. Genome 35, 385–391 (1992)CrossRefGoogle Scholar
  33. 33.
    Chen, Y.P.: Microwave treatment of eight seconds protects cells of Isafis indigofica from enhanced UV-8 radiation lesions. Photochem Photobiol. 82, 503–507 (2006)Google Scholar
  34. 34.
    Fulda, S., Gorman, A.M., Hori, O., Samali, A.: Cellular stress responses: cell survival and cell death. Intl J Cell Biol. (2010). doi: 10.1155/2010/245803
  35. 35.
    Dumet, D., Grapin, A., Bailly, C., Dorion, N.: Revisiting crucial steps of an encapsulation/desiccation based cryopreservation process: importance of thawing method in the case of Pelargonium meristems. Plant Sci. 163, 1121–1127 (2002)CrossRefGoogle Scholar
  36. 36.
    Engelmann, F.: In vitro conservation methods. In: Callow, J.A., Ford-Loyd, B.V., Newbury, H.J. (eds.) Biotechnology and Plant Genetic Resources, pp. 119–161. CAB International, Oxford (1997)Google Scholar
  37. 37.
    Thinh, N.T., Takagi, H.: Cryopreservation of in vitro grown apical meristems of terrestrial orchids (Cymbidium spp.) by vitrification. In: Engelmann, F., Takagi, H. (eds.) Cryopreservation of Tropical Plant Germplasm: Current Research Progress and Applications, pp. 441–443. JIRCAS, Tsukuba and IPGRI, Rome (2000)Google Scholar
  38. 38.
    Jayasanka, S.M.D.H., Asaeda, T.: The significance of microwaves in the environment and its effect on plants. Environ Rev. 22, 220–228 (2014)CrossRefGoogle Scholar
  39. 39.
    Arora, A., Sairam, R.K., Srivastava, G.C.: Oxidative stress and antioxidative system in plants. Curr Sci. 82, 1227–1238 (2002)Google Scholar
  40. 40.
    Volkrodt, W.: Droht den Mikrowellen ein ähnliches Fiasko wie der Atomenergie? Wetter-Boden-Mensch. 4, 16-23 (1991)Google Scholar
  41. 41.
    Balodis, V., Brumelis, G., Kalviskis, K., Nikodemus, O., Tjarve, D., Znotina, V.: Does the Skrunda radio location station diminish the radial growth of pine trees? Sci Total Environ. 180, 57–64 (1996)CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  1. 1.National Institute of Research and Development for Biological SciencesBranch Institute of Biological ResearchCluj-NapocaRomania
  2. 2.National Institute of Research and Development for Isotopic and Molecular TechnologiesCluj-NapocaRomania

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