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Influence of individual cell motility on the 2D front roughness dynamics of tumour cell colonies

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Abstract

The dynamics of in situ 2D HeLa cell quasi-linear and quasi-radial colony fronts in a standard culture medium is investigated. For quasi-radial colonies, as the cell population increased, a kinetic transition from an exponential to a constant front average velocity regime was observed. Special attention was paid to individual cell motility evolution under constant average colony front velocity looking for its impact on the dynamics of the 2D colony front roughness. From the directionalities and velocity components of cell trajectories in colonies with different cell populations, the influence of both local cell density and cell crowding effects on individual cell motility was determined. The average dynamic behaviour of individual cells in the colony and its dependence on both local spatio-temporal heterogeneities and growth geometry suggested that cell motion undergoes under a concerted cell migration mechanism, in which both a limiting random walk-like and a limiting ballistic-like contribution were involved. These results were interesting to infer how biased cell trajectories influenced both the 2D colony spreading dynamics and the front roughness characteristics by local biased contributions to individual cell motion. These data are consistent with previous experimental and theoretical cell colony spreading data and provide additional evidence of the validity of the Kardar-Parisi-Zhang equation, within a certain range of time and colony front size, for describing the dynamics of 2D colony front roughness.

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Acknowledgments

This work was supported by the Consejo Nacional de Investigaciones Cientficas y Técnicas of Argentina (PIP 2231). P.H.G. thanks the Comisión de Investigaciones Científicas (CIC), Pcia. Bs. As., for financial support. We acknowledge Silvia Coronato for technical assistance. N.E.M. thanks the Universidad Nacional de La Plata and the Ministerio Nacional de Educación for the scholarship from the program ‘Estímulo a las Vocaciones Científicas’.

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  1. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Universidad Nacional de La Plata (UNLP), CONICET, Sucursal 4, Casilla de Correo 16, 1900, La Plata, Argentina

    N. E. Muzzio, M. A. Pasquale & A. J. Arvia

  2. Cátedra de Patología, Facultad de Ciencias Médicas, UNLP, CIC, Calle 60 y 120, 1900, La Plata, Bs. As., Argentina

    P. H. González

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  1. N. E. Muzzio
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  2. M. A. Pasquale
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Correspondence to M. A. Pasquale.

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Muzzio, N.E., Pasquale, M.A., González, P.H. et al. Influence of individual cell motility on the 2D front roughness dynamics of tumour cell colonies. J Biol Phys 40, 285–308 (2014). https://doi.org/10.1007/s10867-014-9349-9

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