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Optical method for monitoring of photodynamic inactivation of bacteria

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Abstract

Photodynamic inactivation is a new promising approach to treat bacterial infections. Usually, the evaluation of the efficacy of this method is done through time-consuming and labor-intensive microbiological test methods. This paper describes the development and implementation of an optical method to evaluate the photodynamic inactivation of bacteria based on non-invasive diffuse reflectance measurements. Five Staphylococcus aureus cultures and 15 mice have been used in this study. A skin lesion was created on the back of all animals, and it was contaminated with S. aureus (5.16 ± 0.013 log CFU/ml). Toluidine Blue O (c = 8.67 × 10 − 3 M) has been used as a photosensitiser agent. The bacterial cultures and animals were exposed to laser radiation (λ = 635 nm, P = 15 mW, DE = 8.654 J/cm2) for 20 min. The photodynamic inactivation of bacteria was monitored by acquiring the wounds’ reflection spectra at different time points and by microbiological exams on the bioptical material. The good correlation between the diffuse reflectance and colony-forming units demonstrates the value of this optical method based on diffuse reflectance measurements as a rapid technique to monitor photodynamic bacterial inactivation.

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Acknowledgements

We thank Prof Dr. Iulian Togoe from Microbiological Department of the University of Agriculture and Veterinary Medicine, Bucharest, Romania for excellent biological assistance. This work was financed by the Ministry of Education, Research, Youth and Sports by CEEX Grant No. 113.

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Authors and Affiliations

  1. National Institute of Research and Development for Optoelectronics INOE 2000, 409 Atomistilor Street, PO BOX MG5, Magurele, Ilfov, 077125, Romania

    Mihaela Antonina Calin

  2. National Institute of Research and Development for Chemistry and Petrochemistry ICECHIM, 202 Independentei Street, Bucharest, Romania

    Rodica Mariana Ion

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  1. Mihaela Antonina Calin
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  2. Rodica Mariana Ion
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Correspondence to Mihaela Antonina Calin.

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Calin, M.A., Ion, R.M. Optical method for monitoring of photodynamic inactivation of bacteria. J Biol Phys 37, 107–116 (2011). https://doi.org/10.1007/s10867-010-9202-8

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  • DOI: https://doi.org/10.1007/s10867-010-9202-8

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