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Complexation of Biologically Active Aromatic Compounds with DNA in the Presence of Theophylline

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Abstract

1H NMR measurements (500 MHz) have been used to determine the equilibrium hetero-association constants of theophylline (THP) with various biologically active aromatic compounds (daunomycin, novantrone, ethidium bromide, proflavine, norfloxacin) and the complexation constants of THP with both single- and double-stranded oligonucleotides in solution. The results provide a quantitative estimation of the effect of THP on the binding of aromatic ligands with DNA, and a determination of the fraction of aromatic ligand removed from DNA on addition of THP.

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Authors and Affiliations

  1. Faculty of Chemical Sciences, Autonomous University of Puebla, Puebla, Mexico

    A. A. Hernandez Santiago & A. M. Cervantes Tavera

  2. Department of Physics, Sevastopol National Technical University, Universitetskaya str., 33, Sevastopol, 99053, Ukraine

    D. D. Andrejuk & M. P. Evstigneev

  3. School of Biological and Chemical Sciences, Birkbeck College, University of London, London, UK

    D. B. Davies

Authors
  1. A. A. Hernandez Santiago
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  2. D. D. Andrejuk
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  3. A. M. Cervantes Tavera
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  4. D. B. Davies
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  5. M. P. Evstigneev
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Correspondence to M. P. Evstigneev.

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Hernandez Santiago, A.A., Andrejuk, D.D., Cervantes Tavera, A.M. et al. Complexation of Biologically Active Aromatic Compounds with DNA in the Presence of Theophylline. J Biol Phys 35, 115–126 (2009). https://doi.org/10.1007/s10867-008-9124-x

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  • DOI: https://doi.org/10.1007/s10867-008-9124-x

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