Abstract
The C57BL/6 mouse strain have been commonly used for the genetic background animal models and experimental research. There are several major sources of C57BL/6 substrains for the biomedical research community which display genetic and phenotypic differences. Previous studies have suggested that the varies in baseline of cardiovascular phenotypes as well as in response to pressure overload by transverse aortic constriction (TAC). To investigate whether there exist substrain specific differences in response to heart failure post myocardial infarction (MI), consequently the impaired mitochondrial respiration, we performed MI surgery on two commonly used C57BL/6 substrains: C57BL/6J (BL/6J) and C57BL/6NCrl (BL/6N) mice. Subsequently, measurements about cardiac function, histology and mitochondrial respiration capacities were conducted to evaluate the differences. The data showed that C57BL/6J(BL/6J) mice is more resistant to the attack of MI, evidenced by lower mortality, less infarct size and better preserved cardiac function after MI, especially exhibited better mitochondrial respiration capacities, compared with the C57BL/6NCrl(BL/6N) mice.
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The datasets generated and analysed during the current study are available from the corresponding author on reasonable request.
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This work was supported by grants from the National Natural Science Foundation of China (81500312). The funding sponsors played no role in study design, data collection and analysis, interpretation, writing of the report, and the decision to submit the paper for publication. The authors declare that they have no competing interests.
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All animal experiments were approved by the Animal Research Committee of Tongji Medical College, Huazhong Science and Technology University, China.
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Zhou, Z., Liu, Z., Gao, X. et al. Mitochondrial respiration in C57BL/6 substrains varies in response to myocardial infarction. J Bioenerg Biomembr 53, 119–127 (2021). https://doi.org/10.1007/s10863-021-09884-6
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DOI: https://doi.org/10.1007/s10863-021-09884-6