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Study of the properties of doxorubicin-resistant cells affected by acute leucosis

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Abstract

The stiffness of cell membrane was found to be one of the factors determining resistance of a cell in vitro to antibiotic doxorubicin action. Membranes of surviving cells are negatively charged (−35 – −30 mV) and have high values of stiffness (2.2–5.1 μРа) at the doxorubicin concentrations in the medium of 1–500 μg/ml. If the drug concentration and exposure time are being increased, only cells with ‘soft’ membrane (0.25–1 μРа) and positive surface potential (15–29 mV) survive. The data obtained have important prognostic value in studying drug resistance of tumour blood cells and can be used as objective markers of efficiency of the antitumor therapy.

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Acknowledgements

We thank the Russian Science Foundation (project No. 14-14-00328) for financial support and also appreciate the participation of anonymous donors.

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Correspondence to Marina Yu. Skorkina or Anatoly S. Buchelnikov.

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The local ethic committee at the Medical Institute of Belgorod State University approved the present study. The consciously agreements were obtained from all the patients according to the recommendations of the Declaration of Helsinki (The International Response to Helsinki VI, The WMA’s Declaration of Helsinki on Ethical Principles for Medical Research Involving Human Subjects, as adopted by the 52nd WMA General Assembly, Edinburgh, October, 2000).

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Skorkina, M.Y., Shamray, E.A., Salo, V.A. et al. Study of the properties of doxorubicin-resistant cells affected by acute leucosis. J Bioenerg Biomembr 50, 53–58 (2018). https://doi.org/10.1007/s10863-017-9738-4

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  • DOI: https://doi.org/10.1007/s10863-017-9738-4

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