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Altered cellular membrane fluidity levels and lipid peroxidation during experimental pancreas transplantation

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Abstract

Although the pathogenesis of ischemia reperfusion (IR) injury is based on complex mechanisms, free radicals play a central role. We evaluated membrane fluidity and lipid peroxidation during pancreas transplantation (PT) performed in 12 pigs (six donors and six recipients). Fluidity was measured by fluorescence spectroscopy, and malondialdehyde (MDA) and 4-hydroxyalkenals (4-HDA) concentrations were used as an index of lipid oxidation. Pancreatic tissues were collected as follows: (A) donor, immediately before vascular clamping; (B) graft, following perfusion lavage with University of Wisconsin preservation fluid; (C) graft, after 16 h of cold ischemia; and (D) recipient, 30 min vascular postreperfusion. Fluidity and MDA and 4-HDA concentrations were similar in cases A, B, and C. However, there was significant membrane rigidity and increased lipid peroxidation after reperfusion (D). These findings suggest that reperfusion exaggerates oxidative damage and may account for the rigidity in the membranes of allografts during PT.

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Authors and Affiliations

  1. Department of Surgery, Gynaecology and Obstetrics, University of Zaragoza, Zaragoza, Spain

    F. A. García-Gil, P. Royo-Dachary, J. Martínez-Guillén & M. Martínez-Díez

  2. Department of Pharmacology and Physiology, University of Zaragoza, Zaragoza, Spain

    C. D. Albendea, E. Piedrafita & J. J. García

  3. Department of Human Anatomy and Histology, University of Zaragoza, Zaragoza, Spain

    L. López-Pingarrón

  4. Department of Pathological Anatomy, University of Zaragoza, Zaragoza, Spain

    J. Soria

  5. Departamento de Farmacología y Fisiología, Facultad de Medicina de Zaragoza, C/ Domingo Miral s/n, 50009, Zaragoza, Spain

    J. J. García

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  1. F. A. García-Gil
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  2. C. D. Albendea
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  3. L. López-Pingarrón
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García-Gil, F.A., Albendea, C.D., López-Pingarrón, L. et al. Altered cellular membrane fluidity levels and lipid peroxidation during experimental pancreas transplantation. J Bioenerg Biomembr 44, 571–577 (2012). https://doi.org/10.1007/s10863-012-9459-7

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