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Physiological function of soluble cytochrome c-552 from alkaliphilic Pseudomonas alcaliphila AL15-21T

Journal of Bioenergetics and Biomembranes volume 43, Article number: 473 (2011) Cite this article

Abstract

It has been found that the alkaliphilic Gram-negative bacterium Pseudomonas alcaliphila AL15-21T produces a larger amount of soluble c-type cytochromes at pH 10.0 under air-limited condition than at pH 7.0 under high aeration. Cytochrome c-552 was confirmed as the major c-type cytochrome among three soluble c-type cytochromes in the strain. To understand the physiological function of cytochrome c-552, a P. alcaliphila AL15-21T cytochrome c-552 gene deletion mutant without a marker gene was constructed by electrotransformation adjusted in this study for the strain. The maximum specific growth rate and maximum cell turbidity of cells grown at pHs 7.0 and 10.0 under the high-aeration condition did not differ significantly between the wild-type and cytochrome c-552 deletion mutant strains. In the mutant grown at pH 10.0 under low-aeration condition, marked decreases in the maximum specific growth rate (40%) and maximum cell turbidity (25%) compared with the wild type were observed. On the other hand, the oxygen consumption rates of cell suspensions of the mutant obtained by the growth at pH 10 under low-aeration condition were slightly higher than that of the wild type. Considering the high electron-retaining ability of cytochrome c-552, the above observations could be accounted for by cytochrome c-552 acting as an electron sink in the periplasmic space. This may facilitate terminal oxidation in the respiratory system at high pH under air-limited conditions.

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Author notes

  1. Toshihede Matsuno

    Present address: Department of Bioscience and Technology, College of Life Science, Ritsumeikan University, Nojihigashi 1-1-1, Kusatus, Shiga, 525-8577, Japan

  2. Kazuaki Yoshimune

    Present address: Department of Applied Molecular Chemistry, College of Industrial Technology, Nihon University, 1-2-1, Izumichou, Narashino, Chiba, 275-8575, Japan

Affiliations

  1. Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1, Tsukisamu-Higashi, Toyohira-ku, Sapporo, 062-8517, Japan

    Toshihede Matsuno, Kazuaki Yoshimune & Isao Yumoto

  2. Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kita-ku, Sapporo, 060-8589, Japan

    Toshihede Matsuno & Isao Yumoto

Authors
  1. Toshihede Matsuno
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  2. Kazuaki Yoshimune
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  3. Isao Yumoto
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Correspondence to Isao Yumoto.

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Matsuno, T., Yoshimune, K. & Yumoto, I. Physiological function of soluble cytochrome c-552 from alkaliphilic Pseudomonas alcaliphila AL15-21T . J Bioenerg Biomembr 43, 473 (2011). https://doi.org/10.1007/s10863-011-9376-1

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  • DOI: https://doi.org/10.1007/s10863-011-9376-1

Keywords

  • Alkaliphilic
  • Pseudomonas alcaliphila
  • Cytochrome c 5