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Journal of Bioenergetics and Biomembranes

, Volume 41, Issue 3, pp 289–297 | Cite as

Abundant expression and purification of biologically active mitochondrial citrate carrier in baculovirus-infected insect cells

  • Marianna Madeo
  • Chiara Carrisi
  • Domenico Iacopetta
  • Loredana Capobianco
  • Anna Rita Cappello
  • Cecilia Bucci
  • Ferdinando Palmieri
  • Giancarlo Mazzeo
  • Anna Montalto
  • Vincenza Dolce
Article

Abstract

Heterologous expression of recombinant proteins is an essential technology for protein characterization. A major obstacle to investigating the biochemical properties of membrane proteins is the difficulty in obtaining sufficient amounts of functional protein. Here we report the successful expression of the tricarboxylate (or citrate) carrier (CIC) of eel (Anguilla anguilla) from Spodoptera frugiperda (Sf9) cells using the baculovirus expression system. The recombinant CIC was purified by affinity chromatography on Ni2+-NTA agarose; the yield of the purified active protein was 0.4–0.5 mg/l of culture. The transport characteristics of the recombinant CIC and the effects of inhibitors on transport are similar to those determined for eel liver mitochondrial CIC. Because the CIC is one member of an extensive family of mitochondrial transport proteins, it is likely that the procedure used in this study to express and purify this carrier can be successfully applied to other mitochondrial transport proteins, thus providing sufficient protein for functional characterization.

Keywords

Mitochondria Citrate carrier Membrane protein Detergents V5/His-tag Baculovirus expression 

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Marianna Madeo
    • 1
  • Chiara Carrisi
    • 2
  • Domenico Iacopetta
    • 1
  • Loredana Capobianco
    • 2
  • Anna Rita Cappello
    • 1
  • Cecilia Bucci
    • 2
  • Ferdinando Palmieri
    • 3
    • 4
  • Giancarlo Mazzeo
    • 1
  • Anna Montalto
    • 1
  • Vincenza Dolce
    • 1
  1. 1.Department of Pharmaco-BiologyUniversity of CalabriaCosenzaItaly
  2. 2.Department of Biological and Environmental Sciences and TechnologiesUniversity of SalentoLecceItaly
  3. 3.Department of Pharmaco-BiologyUniversity of BariBariItaly
  4. 4.CNR Institute of Biomembranes and BioenergeticsBariItaly

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