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Journal of Bioenergetics and Biomembranes

, Volume 37, Issue 6, pp 441–444 | Cite as

Zooming in on ATP Hydrolysis in F1

  • Markus Dittrich
  • Klaus Schulten
Article

 

We summarize our current view of the reaction mechanism in F1-ATPase as it has emerged from experiment, theory, and computational studies over the last several years. ATP catalysis in the catalytic binding pockets of F1 takes place without the release of any significant free energy and is efficiently driven by the combined action of two water molecules utilizing a so-called protein-relay mechanism. The chemical reaction itself is controlled by the spatial position of a key arginine residue.

Key Words

F1-ATPase ATP hydrolysis QM/MM molecular mechanics 

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References

  1. Abrahams, J., Leslie, A., Lutter, R., and Walker, J. (1994). Nature 370, 621–628.CrossRefGoogle Scholar
  2. Ahmad, Z., and Senior, A. E. (2004). J. Biol. Chem. 44, 46057–46064.CrossRefGoogle Scholar
  3. Berg, J. M., Tymoczko, J. L., and Stryer, L. (2002). Biochemistry, 5th edn., W. H. Freeman, New York.Google Scholar
  4. Böckmann, R. A., and Grubmüller, H. (2002). Nature Struct. Biol. 9, 198–202.Google Scholar
  5. Dittrich, M., Hayashi, S., and Schulten, K. (2003). Biophys. J. 85, 2253–2266.CrossRefGoogle Scholar
  6. Dittrich, M., Hayashi, S., and Schulten, K. (2004). Biophys. J. 87, 2954–2967.CrossRefGoogle Scholar
  7. Li, G., and Cui, Q. (2004). J. Phys. Chem. B 108, 3342–3357.CrossRefGoogle Scholar
  8. Nishizaka, T., Oiwa, K., Noji, H., Kimura, S., Muneyuki, E., Yoshida, M., and Kinosita, K., Jr. (2004). Nat. Struct. Mol. Biol. 11, 142–148.CrossRefGoogle Scholar
  9. Oster, G., and Wang, H. (2000). Biochim. Biophys. Acta 1458, 482–510.CrossRefGoogle Scholar
  10. Scheidig, A. J., Burmester, C., and Goody, R. S. (1999). Structure 7, 1311–1324.CrossRefGoogle Scholar
  11. Shimabukuro, K., Yasuda, R., Muneyuki, E., Hara, K. Y., Kinosita, K., Jr., and Yoshida, M. (2003). Proc. Natl. Acad. Sci. U.S.A. 100, 14731–14736.Google Scholar
  12. Strajbl, M., Shurki, A., and Warshel, A. (2003). Proc. Natl. Acad. Sci. U.S.A. 100, 14834–14839.Google Scholar
  13. Weber, J., Nadanaciva, S., and Senior, A. E. (2000). FEBS Lett. 483, 1–5.CrossRefGoogle Scholar

Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  1. 1.Beckman InstituteUniversity of Illinois at Urbana–ChampaignUrbana

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