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Journal of Bioenergetics and Biomembranes

, Volume 37, Issue 6, pp 393–398 | Cite as

Structure and Regulation of the V-ATPases

  • Takao Inoue
  • Yanru Wang
  • Kevin Jefferies
  • Jie Qi
  • Ayana Hinton
  • Michael Forgac
Article

 

The V-ATPases are ATP-dependent proton pumps present in both intracellular compartments and the plasma membrane. They function in such processes as membrane traffic, protein degradation, renal acidification, bone resorption and tumor metastasis. The V-ATPases are composed of a peripheral V1 domain responsible for ATP hydrolysis and an integral V0 domain that carries out proton transport. Our recent work has focused on structural analysis of the V-ATPase complex using both cysteine-mediated cross-linking and electron microscopy. For cross-linking studies, unique cysteine residues were introduced into structurally defined sites within the B and C subunits and used as points of attachment for the photoactivated cross-linking reagent MBP. Disulfide mediated cross-linking has also been used to define helical contact surfaces between subunits within the integral V0 domain. With respect to regulation of V-ATPase activity, we have investigated the role that intracellular environment, luminal pH and a unique domain of the catalytic A subunit play in controlling reversible dissociation in vivo.

Key Words

vacuolar (H+)-ATPase membrane protein structure pH regulation yeast 

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Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Takao Inoue
    • 1
  • Yanru Wang
    • 1
  • Kevin Jefferies
    • 1
  • Jie Qi
    • 1
  • Ayana Hinton
    • 1
  • Michael Forgac
    • 1
  1. 1.Department of PhysiologyTufts University School of MedicineBoston

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