Journal of Bioenergetics and Biomembranes

, Volume 37, Issue 6, pp 381–386 | Cite as

Splice Variants of the Gamma Subunit (FXYD2) and Their Significance in Regulation of the Na, K-ATPase in Kidney

  • Elena Arystarkhova
  • Kathleen J. Sweadner


The recent discovery of a family of single-span membrane proteins (the FXYD proteins) introduced a new direction to the rather complicated area of regulation of Na, K-ATPase. At least six members of the family have been shown to associate with the Na, K-ATPase in a cell- and tissue-specific manner, while four of them, namely the γ subunit (FXYD2), CHIF (FXYD4), phospholemman (FXYD1), and dysadherin (FXYD5) have been identified in kidney. All four exhibited different effects on the properties of the pump in heterologous expression systems. Taken along with their non-overlapping expression patterns in the nephron, this provides a potential structural basis for the segment-specific properties of the Na, K-ATPase that had been reported in a number of papers on kidney physiology.

This brief review summarizes our own contributions on structure/functional characterization of one of the family members, the γ subunit (FXYD2). The focus is on splice variants of γ, their structural similarity and yet distinct effects conferred to Na, K-ATPase.

Key Words

FXYD family endogenous inibitor post-translational modification stress response growth control 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Arystarkhova, E., Donnet, C., Asinovski, N. K., and Sweadner, K. J. (2002a). J. Biol. Chem. 277, 10162–10172.CrossRefGoogle Scholar
  2. Arystarkhova, E., Wetzel, R. K., Asinovski, N. K., and Sweadner, K. J. (1999). J. Biol. Chem. 274, 33183–33185.CrossRefGoogle Scholar
  3. Arystarkhova, E., Wetzel, R. K., and Sweadner, K. J. (2002b). Am. J. Physiol. 282, F393–F407.Google Scholar
  4. Barlet Bas, C., Cheval, L., Khadouri, C., Marsy, S., and Doucet, A. (1990). Am. J. Physiol. 259, F246–F250.Google Scholar
  5. Capasso, J. M., Rivard, C., and Berl, T. (2001). Proc. Natl. Acad. Sci. USA 23, 3414–3419.Google Scholar
  6. Donnet, C., Arystarkhova, E., and Sweadner, K. J. (2001). J. Biol. Chem. 276, 7357–7365.CrossRefGoogle Scholar
  7. Jones, D. H., Li, T. Y., Arystarkhova, E., Barr, K. J., Wetzel, R. K., Peng, J., Markham, K., Sweadner, K. J., Fong, G.-H., and Kidder, G. M. (2005). J. Biol. Chem. 280, 19003–19011.CrossRefGoogle Scholar
  8. Kassed, C. A., Butler, T. L., Patton, G. W., Demesquita, D. D., Navidomskis, M. T., Memet, S., Israel, A., and Pennypacker, K. R. (2004). FASEB J. 18, 723–724.Google Scholar
  9. Küster, B., Shainskaya, A., Pu, H. X., Goldshleger, R., Blostein, R., and Karlish, S. J. D. (2000). J. Biol. Chem. 275, 18441–18446.CrossRefGoogle Scholar
  10. Sweadner, K. J., Wetzel, R. K., and Arystarkhova, E. (2000). Biochem. Biophys. Res. Commun. 279, 196–201.CrossRefGoogle Scholar
  11. Wetzel, R. K., Pascoa, J. L., and Arystarkhova, E. (2004). J. Biol. Chem. 279, 41750–41757.CrossRefGoogle Scholar

Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Elena Arystarkhova
    • 1
    • 2
  • Kathleen J. Sweadner
    • 1
  1. 1.Laboratory of Membrane BiologyMassachusetts General Hospital and Harvard Medical SchoolBoston
  2. 2.415 Thier (Wellman) Research BuildingMassachusetts General HospitalBoston

Personalised recommendations