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High sensitivity high-resolution full range relaxometry using a fast mechanical sample shuttling device and a cryo-probe

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Field-dependent NMR studies of bio-molecular systems using a sample shuttling hardware operating on a high-field NMR apparatus have provided valuable structural and dynamic information. We have recently published a design of a compact sample transportation device, called “field-cycler”, which was installed in a commercial spectrometer and which provided highly precise positioning and stability during high speed shuttling. In this communication, we demonstrate the first use of a sample shuttling device on a commercial high field standard bore NMR spectrometer, equipped with a commercial triple resonance cryogenically cooled NMR probe. The performance and robustness of the hardware operating in 1D and 2D field cycling experiments, as well as the impact of the sample shuttling time on the signal intensity are discussed.

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We greatly acknowledge Dr. Sophie Zinn-Justin (CEA) for stimulating discussions and for allowing us access to the 700 MHz spectrometer, Dr. Nikolas Birlirakis (ENS) for useful comments on the manuscript and Dr. F. Ferrage (ENS) for comments and suggestions. We would like to give the special acknowledgement to Dr. Jan Marchant and the labs of Prof. Michael F. Summers and Prof. Kwaku T. Dayie in Maryland University, USA, for the UUCG tetra-loop RNA sample preparation. We also thank Mr. Angelo Guiga and the staffs in the Academia Sinica Machine Shop, Taiwan, especially Mr. Cherng-Yin Lin, for the production of the high-precision hardware. This work is supported by ERC grants 2F4BIODYN and R-EVOLUTION-M-R (StG 205119), ANR DYN-IDP in France, and NSC 102-2113-M-001-010 from The National Science Council, The Republic of China.

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Correspondence to Tai-huang Huang.

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Chou, CY., Chu, M., Chang, CF. et al. High sensitivity high-resolution full range relaxometry using a fast mechanical sample shuttling device and a cryo-probe. J Biomol NMR 66, 187–194 (2016).

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