Journal of Biomolecular NMR

, Volume 61, Issue 2, pp 151–160 | Cite as

Perspectives for sensitivity enhancement in proton-detected solid-state NMR of highly deuterated proteins by preserving water magnetization

  • Veniamin ChevelkovEmail author
  • ShengQi Xiang
  • Karin Giller
  • Stefan Becker
  • Adam Lange
  • Bernd Reif


In this work, we show how the water flip-back approach that is widely employed in solution-state NMR can be adapted to proton-detected MAS solid-state NMR of highly deuterated proteins. The scheme allows to enhance the sensitivity of the experiment by decreasing the recovery time of the proton longitudinal magnetization. The method relies on polarization transfer from non-saturated water to the protein during the inter-scan delay.


Magic angle spinning (MAS) solid-state NMR Perdeuteration Sensitivity enchancement Protein water interaction 



We thank Brigitta Angerstein for expert technical assistance. This work was supported by the Max Planck Society, the Leibniz-Gemeinschaft, and the DFG (Emmy Noether Fellowship to A.L). A.L. and S.X. acknowledge funding from the CRC803 (DFG).

Supplementary material

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Supplementary material 1 (DOC 1781 kb)
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Supplementary material 2 (PDF 17 kb)
10858_2015_9902_MOESM3_ESM.pdf (14 kb)
Supplementary material 3 (PDF 14 kb)


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Copyright information

© European Union 2015

Authors and Affiliations

  • Veniamin Chevelkov
    • 1
    • 2
    Email author
  • ShengQi Xiang
    • 1
  • Karin Giller
    • 1
  • Stefan Becker
    • 1
  • Adam Lange
    • 1
    • 2
    • 3
  • Bernd Reif
    • 4
    • 5
  1. 1.Max-Planck-Institut für biophysikalische Chemie (MPI-bpc)GoettingenGermany
  2. 2.Leibnizinstitut für Molekulare Pharmakologie (FMP)BerlinGermany
  3. 3.Institut für BiologieHumboldt Universität zu BerlinBerlinGermany
  4. 4.Munich Center for Integrated Protein Science (CIPS-M), Department ChemieTechnische Universität München (TUM)GarchingGermany
  5. 5.Helmholtz-Zentrum München (HMGU), Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU)NeuherbergGermany

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