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UTOPIA NMR: activating unexploited magnetization using interleaved low-gamma detection

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Abstract

A growing number of nuclear magnetic resonance (NMR) spectroscopic studies are impaired by the limited information content provided by the standard set of experiments conventionally recorded. This is particularly true for studies of challenging biological systems including large, unstructured, membrane-embedded and/or paramagnetic proteins. Here we introduce the concept of unified time-optimized interleaved acquisition NMR (UTOPIA-NMR) for the unified acquisition of standard high-γ (e.g. 1H) and low-γ (e.g. 13C) detected experiments using a single receiver. Our aim is to activate the high level of polarization and information content distributed on low-γ nuclei without disturbing conventional magnetization transfer pathways. We show that using UTOPIA-NMR we are able to recover nearly all of the normally non-used magnetization without disturbing the standard experiments. In other words, additional spectra, that can significantly increase the NMR insights, are obtained for free. While we anticipate a broad range of possible applications we demonstrate for the soluble protein Bcl-xL (ca. 21 kDa) and for OmpX in nanodiscs (ca. 160 kDa) that UTOPIA-NMR is particularly useful for challenging protein systems including perdeuterated (membrane) proteins.

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Acknowledgments

The authors acknowledge access to the Jülich-Düsseldorf Biomolecular NMR Center. We thank Eriks Kupce for helpful discussions. This work was supported by Grants from the German Academic Exchange Service (DAAD) and the German Research Foundation (DFG) (ET 103/2-1) to M.E. and from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie Grant Agreement No. 660258 to A.V.. G.W. acknowledges support by National Institutes of Health (NIH) Grants GM047467, GM075879. Acquisition and maintenance of instruments used for this research were supported by NIH Grants EB002026 and S10 RR026417.

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Author notes

  1. Tsyr-Yan Yu

    Present address: Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei, Taiwan

Authors and Affiliations

  1. Institute of Physical Biology, Heinrich-Heine-University, Düsseldorf, Germany

    Aldino Viegas, Thibault Viennet & Manuel Etzkorn

  2. Instititue of Complex Systems, Forschungszentrum Jülich, Jülich, Germany

    Thibault Viennet & Manuel Etzkorn

  3. Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA

    Tsyr-Yan Yu & Gerhard Wagner

  4. Bruker BioSpin GmbH, Rheinstetten, Germany

    Wolfgang Bermel

  5. Bruker BioSpin GmbH, Fällanden, Switzerland

    Frank Schumann

Authors
  1. Aldino Viegas
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  2. Thibault Viennet
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  3. Tsyr-Yan Yu
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  4. Frank Schumann
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  5. Wolfgang Bermel
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  6. Gerhard Wagner
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  7. Manuel Etzkorn
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Correspondence to Manuel Etzkorn.

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Additional information

The pulse sequences used in this manuscript are beta-versions that require experienced users for their implementation. Until more user-friendly versions are released (on the corresponding authors homepage as well as in the Bruker library) please directly contact Dr. Manuel Etzkorn (manuel.etzkorn@hhu.de) to obtain the beta-versions as well as instructions/support for setting them up.

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Viegas, A., Viennet, T., Yu, TY. et al. UTOPIA NMR: activating unexploited magnetization using interleaved low-gamma detection. J Biomol NMR 64, 9–15 (2016). https://doi.org/10.1007/s10858-015-0008-7

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