Abstract
13C-α-ketoacid metabolic precursors of phenylalanine and tyrosine effectively enter the metabolism of a protein overexpressing E. coli strain to label Phe- and Tyr-residues devoid of any cross-labelling. The methodology gives access to highly selective labelling patterns as valuable tools in protein NMR spectroscopy without the need of 15N-chiral amino acid synthesis using organic chemistry.
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This work was supported in part by the FWF (P20549-N19 and W-1221-B03 to R.K.).
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Lichtenecker, R.J., Weinhäupl, K., Schmid, W. et al. α-Ketoacids as precursors for phenylalanine and tyrosine labelling in cell-based protein overexpression. J Biomol NMR 57, 327–331 (2013). https://doi.org/10.1007/s10858-013-9796-9
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DOI: https://doi.org/10.1007/s10858-013-9796-9