Abstract
We describe here, adaptation of the HNN pulse sequence for multiple nuclei detection using two independent receivers by utilizing the detectable 13Cα transverse magnetization which was otherwise dephased out in the conventional HNN experiment. It enables acquisition of 2D 13Cα–15N sequential correlations along with the standard 3D 15N–15N–1H correlations, which provides directionality to sequential walk in HNN, on one hand, and enhances the speed of backbone assignment, on the other. We foresee that the implementation of dual direct detection opens up new avenues for a wide variety of modifications that would further enhance the value and applications of the experiment, and enable derivation of hitherto impossible information.
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Acknowledgments
We thank the Government of India for providing financial support to the National Facility for High Field NMR at TIFR. We like to express our sincere gratitude to Dr. P. K. Madhu for his critical suggestions.
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Swagata Chakraborty and Subhradip Paul contributed equally.
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Chakraborty, S., Paul, S. & Hosur, R.V. Simultaneous acquisition of 13Cα–15N and 1H–15N–15N sequential correlations in proteins: application of dual receivers in 3D HNN. J Biomol NMR 52, 5–10 (2012). https://doi.org/10.1007/s10858-011-9596-z
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DOI: https://doi.org/10.1007/s10858-011-9596-z