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Exclusively NOESY-based automated NMR assignment and structure determination of proteins

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Abstract

A fully automated method is presented for determining NMR solution structures of proteins using exclusively NOESY spectra as input, obviating the need to measure any spectra only for obtaining resonance assignments but devoid of structural information. Applied to two small proteins, the approach yielded structures that coincided closely with conventionally determined structures.

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Acknowledgments

We thank Drs. H. Yoshida, T. Terauchi, and A. M. Ono for preparing the SAIL ubiquitin sample. Financial support by a Grant-in-Aid for Scientific Research of the Japan Society for the Promotion of Science, the Targeted Proteins Research Program of the Ministry of Education, Culture, Sports, Science and Technology of Japan, and the Lichtenberg program of the Volkswagen Foundation is gratefully acknowledged.

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Authors and Affiliations

  1. Institute of Biophysical Chemistry, Center for Biomolecular Magnetic Resonance, and Frankfurt Institute for Advanced Studies, Goethe University Frankfurt am Main, Max-von-Laue-Str. 9, 60438, Frankfurt am Main, Germany

    Teppei Ikeya & Peter Güntert

  2. Graduate School of Science, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo, 192-0397, Japan

    Teppei Ikeya, Jun-Goo Jee, Yoshiki Shigemitsu, Junpei Hamatsu, Masaki Mishima, Yutaka Ito, Masatsune Kainosho & Peter Güntert

  3. Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8602, Japan

    Masatsune Kainosho

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  1. Teppei Ikeya
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  2. Jun-Goo Jee
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Correspondence to Peter Güntert.

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Ikeya, T., Jee, JG., Shigemitsu, Y. et al. Exclusively NOESY-based automated NMR assignment and structure determination of proteins. J Biomol NMR 50, 137–146 (2011). https://doi.org/10.1007/s10858-011-9502-8

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  • DOI: https://doi.org/10.1007/s10858-011-9502-8

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