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Solution NMR structure determination of proteins revisited

  • Perspective
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Abstract

This ‘Perspective’ bears on the present state of protein structure determination by NMR in solution. The focus is on a comparison of the infrastructure available for NMR structure determination when compared to protein crystal structure determination by X-ray diffraction. The main conclusion emerges that the unique potential of NMR to generate high resolution data also on dynamics, interactions and conformational equilibria has contributed to a lack of standard procedures for structure determination which would be readily amenable to improved efficiency by automation. To spark renewed discussion on the topic of NMR structure determination of proteins, procedural steps with high potential for improvement are identified.

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Notes

  1. Quotation from the “Report of the Protein Structure Initiative Assessment Panel” (http://www.nigms.nih.gov/News/Reports/PSIAssessmentPanel2007.htm). “A.3.2C NMR. NMR technology from the PSI is not seen as having the general impact described above for crystallography. Many of the NMR advances (G-matrix Fourier-transform NMR, automated data analysis and structure determination) are not readily applicable to complex systems with higher molecular weight. Thus, these have not changed the practices of the NMR community in the way PSI-supported technologies have changed the crystallographic community.”

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Acknowledgements

We thank Dr. Ian A. Wilson for a critical reading of the manuscript. M.B. acknowledges support by the EU (LSHG-CT-2005-018988), G.W. by the NIH grant GM 47467, and K.W. by the Joint Center for Structural Genomics in La Jolla, CA, USA (JCSG, NIH #GM074898) and the NCCR Structural Biology (Swiss National Science Foundation and ETH Zürich, Switzerland).

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Correspondence to Martin Billeter.

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Billeter, M., Wagner, G. & Wüthrich, K. Solution NMR structure determination of proteins revisited. J Biomol NMR 42, 155–158 (2008). https://doi.org/10.1007/s10858-008-9277-8

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  • DOI: https://doi.org/10.1007/s10858-008-9277-8

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