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SWET for Secure Water Suppression on Probes with High Quality Factor

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Abstract

Water suppression by selective preirradiation is increasingly difficult to achieve on probeheads with high quality factor because of the opposing forces of radiation damping. Here we show that a simple modification to the WET scheme provides reliable water suppression in aqueous solutions of proteins and peptides with minimal saturation of the Hα protons. The scheme is shown to work also with dilute peptide solutions. It is recommended to maintain the water suppression during the evolution time of COSY experiments by weak selective irradiation that causes only minimal Bloch-Siegert shifts. The new water-suppression scheme suppresses the water magnetization by spatial scrambling. Traditional water suppression by preirradiation is similarly based more on water scrambling due to the radiofrequency inhomogeneity than on relaxation effects.

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Abbreviations

DANTE:

delays alternating with nutations for tailored excitation

rf:

radiofrequency

SWET:

secure WET

WANTED:

Water selective DANTE using gradient

WET:

water suppression enhanced through T1 effects

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Authors and Affiliations

  1. Research School of Chemistry, Australian National University, Canberra, ACT, 0200, Australia

    Peter S. C. Wu & Gottfried Otting

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  1. Peter S. C. Wu
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  2. Gottfried Otting
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Correspondence to Gottfried Otting.

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Wu, P.S.C., Otting, G. SWET for Secure Water Suppression on Probes with High Quality Factor. J Biomol NMR 32, 243–250 (2005). https://doi.org/10.1007/s10858-005-8531-6

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