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A Thorough Dynamic Interpretation of Residual Dipolar Couplings in Ubiquitin

Journal of Biomolecular NMR volume 34, pages 101–115 (2006)Cite this article

Abstract

The presence of slow motions with large amplitudes, as detected by measurements based on residual dipolar couplings [Peti, W., Meiler, J., Brueschweiler, R. and Griesinger, C. (2002) J. Am. Chem. Soc., 124, 5822–5833], has stirred up much discussion in recent years. Based on ubiquitin NH residual dipolar couplings (rdcs) measured in 31 different alignment conditions, a model-free analysis of structure and dynamics [Meiler, J., Peti, W., Prompers, J., Griesinger, C. and Brueschweiler, R. (2001) J. Am. Chem. Soc., 123, 6098–6107] is presented. Starting from this broad experimental basis, rdc-based order parameters with so far unattained accuracy were determined. These rdc-based order parameters underpin the presence of new modes of motion slower than the inverse overall tumbling correlation time. Amplitudes and anisotropies of the motion were derived. The effect of structural noise on the results was proven to be negligible.

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Affiliations

  1. NMR based structural biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Goettingen, Germany

    Nils A. Lakomek, Teresa Carlomagno, Stefan Becker & Christian Griesinger

  2. Center of Structural Biology, Department of Chemistry, Vanderbilt University, 465 21st Ave South, Nashville, TN, 37232-8725, USA

    Jens Meiler

Authors
  1. Nils A. Lakomek
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  2. Teresa Carlomagno
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  3. Stefan Becker
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  4. Christian Griesinger
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  5. Jens Meiler
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Correspondence to Christian Griesinger.

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Lakomek, N.A., Carlomagno, T., Becker, S. et al. A Thorough Dynamic Interpretation of Residual Dipolar Couplings in Ubiquitin. J Biomol NMR 34, 101–115 (2006). https://doi.org/10.1007/s10858-005-5686-0

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  • DOI: https://doi.org/10.1007/s10858-005-5686-0

Keywords

  • dynamical averaging
  • model free approach
  • nuclear magnetic resonance (NMR)
  • residual dipolar couplings (rdcs)
  • ubiquitin