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13C-13C NOESY: A constructive use of 13C-13C spin-diffusion

Journal of Biomolecular NMR volume 30, pages 245–251 (2004)Cite this article

Abstract

13C-13C NOESY experiments were performed under long mixing time conditions on reduced human superoxide dismutase (32 kDa, 15N, 13C and 70% 2H labeled). 13C-13C couplings were successfully eliminated through post-processing of in-phase-anti-phase (IPAP) data. It appears that at mixing time τm of 3.0 s the spin diffusion mechanism allows the detection of 96% of the two-bond correlations involving C′ and Cβ. The interpretation was confirmed by simulations. This approach broadens the range of applicability of 13C-13C NOESY spectroscopy.

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Affiliations

  1. Magnetic Resonance Center and Department of Chemistry, University of Florence, Italy

    Ivano Bertini, Isabella C. Felli & Roberta Pierattelli

  2. Bruker BioSpin AG, Fällanden, Switzerland

    Rainer Kümmerle

  3. Magnetic Resonance Center and Department of Agricultural Biotechnology, University of Florence, Italy

    Claudio Luchinat

Authors
  1. Ivano Bertini
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  2. Isabella C. Felli
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  3. Rainer Kümmerle
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  4. Claudio Luchinat
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  5. Roberta Pierattelli
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Correspondence to Ivano Bertini.

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Bertini, I., Felli, I.C., Kümmerle, R. et al. 13C-13C NOESY: A constructive use of 13C-13C spin-diffusion. J Biomol NMR 30, 245–251 (2004). https://doi.org/10.1007/s10858-005-1679-2

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  • DOI: https://doi.org/10.1007/s10858-005-1679-2

Key words

  • 13C direct detection
  • Cu,Zn-SOD
  • NOESY
  • spin diffusion
  • superoxide dismutase