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Coaction of TGF-β1 and CDMP1 in BMSCs-induced laryngeal cartilage repair in rabbits

  • Biomaterials Synthesis and Characterization
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Abstract

Bone marrow mesenchymal stem cells (BMSCs) are well-known for tissue regeneration and bone repair. This study intended to evaluate the potential efficiency BMSCs in poly(lactide-co-glycolide) (PLGA) scaffolds for the treatment of laryngeal cartilage defects. BMSCs were isolated and identified, and added with 10 ng/mL transforming growth factor-beta1 (TGF-β1) or/and 300 ng/mL CDMP1 to coculture with PLGA scaffolds. The chondrogenic differentiation, migration, and apoptosis of BMSCs were detected under the action of TGF-β1 or/and CDMP1. After successful modeling of laryngeal cartilage defects, PLGA scaffolds were transplanted into the rabbits correspondingly. After 8 weeks, laryngeal cartilage defects were assessed. Levels of collagen II, aggrecan, Sox9, Smad2, Smad3, ERK, and JNK were detected. The TGF-β1 or/and CDMP1-induced BMSCs expressed collagen II, aggrecan, and Sox9, with enhanced cell migration and inhibited apoptosis. In addition, laryngeal cartilage defect in rabbits with TGF-β1 or/and CDMP1 was alleviated, and levels of specific cartilage matrix markers were decreased. The combined effects of TGF-β1 and CDMP1 were more significant. The TGF-β1/Smad and ERK/JNK pathways were activated after TGF-β1 or/and CDMP1 were added to BMSCs or rabbits. In summary, BMSCs and PLGA scaffolds repair laryngeal cartilage defects in rabbits by activating the TGF-β1/Smad and ERK/JNK pathways under the coaction of TGF-β1 and CDMP1.

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  1. These authors contributed equally: Linxiang Ma, Yonghong Zhang

Authors and Affiliations

  1. Department of Otolaryngology, Affiliated Hospital of Jining Medical University, Jining, 272029, Shandong, PR China

    Linxiang Ma, Yonghong Zhang & Caihua Wang

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  1. Linxiang Ma
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Correspondence to Caihua Wang.

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This study was approved and supervised by the animal ethics committee of Affiliated Hospital of Jining Medical University. All procedures were strictly conducted in line with the Code of Ethics. Significant efforts were made to minimize the number of animals and their respective suffering.

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Ma, L., Zhang, Y. & Wang, C. Coaction of TGF-β1 and CDMP1 in BMSCs-induced laryngeal cartilage repair in rabbits. J Mater Sci: Mater Med 31, 130 (2020). https://doi.org/10.1007/s10856-020-06454-x

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  • DOI: https://doi.org/10.1007/s10856-020-06454-x

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