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Enhanced osteogenesis of bone marrow stem cells cultured on hydroxyapatite/collagen I scaffold in the presence of low-frequency magnetic field

  • Tissue Engineering Constructs and Cell Substrates
  • Original Research
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Abstract

As a non-invasive biophysical therapy, electromagnetic fields (EMF) have been widely used to promote the healing of fractures. In the present study, hydroxyapatite/collagen I (HAC) loaded with rabbit bone marrow mesenchymal stem cells (MSCs) were cultured in a dynamic perfusion bioreactor and exposed to EMF of 15 Hz/1mT. Osteogenic differentiation of the seeded cells was analyzed through the evaluation of ALP activity and osteogenesis-related genes expression in vitro. The in vivo osteogenesis efficacy of the cell laden HAC constructs treated with/without EMF was evaluated through a rabbit femur condyle defect model. The results showed that EMF of 15 Hz/1mT could enhance the osteogenic differentiation of the cells seeded on HAC scaffold. Furthermore, the in vivo experiments demonstrated that EMF exposure could promote bone regeneration within the defect and bone integration between the graft and host bone. Taking together, the MSCs seeded HAC scaffold combined with EMF exposure could be a promising approach for bone tissue engineering.

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Acknowledgements

This study was supported in part by National Natural Science Foundation of China (No.51877097, No.51537004), Independent Innovative Research Funds of HUST (No.2014QN094) and Research Funds of Tongji Hospital (No. 2017B012).

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Authors and Affiliations

  1. Department of Spine and Spinal Cord Surgery, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, No. 7 Weiwu road, 450003, Zhengzhou, P. R. China

    Huaixi Wang

  2. Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Jiefang Avenue 1095, 430030, Wuhan, P. R. China

    Huaixi Wang, Wenkai Li, Jingyuan Chen, Hao Li, Jiyuan Yan, Xuefeng Yuan, Hua Wu & Chaoxu Liu

  3. Department of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Jiefang Avenue 1095, 430030, Wuhan, P. R. China

    Xiangyu Tang

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Wang, H., Tang, X., Li, W. et al. Enhanced osteogenesis of bone marrow stem cells cultured on hydroxyapatite/collagen I scaffold in the presence of low-frequency magnetic field. J Mater Sci: Mater Med 30, 89 (2019). https://doi.org/10.1007/s10856-019-6289-8

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