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Exploring the effect of sintering temperature on naturally derived hydroxyapatite for bio-medical applications

Journal of Materials Science: Materials in Medicine volume 30, Article number: 21 (2019) Cite this article

Abstract

The current work describes the influence of sintering temperatures on biological and mechanical properties of naturally derived hydroxyapatite (HAp). The phase pure hydroxyapatite developed from the goat bone has been obtained by optimizing the calcination temperature from 600–900 °C. Further, HAp calcined at 900 °C was subjected to various sintering temperature (1100–1400 °C). Finally, the influence of sinter temperatures on mechanical (hardness) and biological properties (in vitro bioactivity, MTT and hemocompatibility assays) were ascertained. In respect of biological properties, it came to know that 1300 °C is optimum sinter temperature, which has enhanced apatite growth with the superior cell viability and hemo-compatible behavior. However, sample sintered at 1400 °C delivers maximum hardness. Thus, the hydroxyapatite extracted from goat bone can find better applications in bio-medical engineering as analogous to the existing man-made synthetic materials.

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References

  1. Wang Q, Chen C, Liu W, He X, Zhou N, Zhang D et al. Levofloxacin loaded mesoporous silica microspheres/nano-hydroxyapatite/polyurethane composite scaffold for the treatment of chronic osteomyelitis with bone defects. Sci Rep. 2017;2:41808.

    Article  Google Scholar 

  2. Lee DJ, Lee YT, Zou R, Daniel R, Ko CC. Polydopamine-laced biomimetic material stimulation of bone marrow derived mesenchymal stem cells to promote osteogenic effects. Sci Rep. 2017;7:12984.

    Article  Google Scholar 

  3. Szczes A, Yan Y, Chibowski E, Hołysz L, Banach M. Properties of natural and synthetic hydroxyapatite and their surface free energy determined by the thin-layer wicking method. Appl Surf Sci. 2018;434:1232–8.

    CAS  Article  Google Scholar 

  4. Mansour SF, El-Dek SI, Ahmed MK. Physico-mechanical and morphological features of zirconia substituted hydroxyapatite nano crystals. Sci Rep. 2017;7:43202.

    CAS  Article  Google Scholar 

  5. Mondal S, Pal U, Dey A. Natural origin hydroxyapatite scaffold as potential bone tissue engineering substitute. Ceram Int. 2016;42:18338–46.

    CAS  Article  Google Scholar 

  6. Heidari F, Bahrololoom ME, Vashaee D, Tayebi L. In situ preparation of iron oxide nanoparticles in natural hydroxyapatite/chitosan matrix for bone tissue engineering application. Ceram Int. 2015;41:3094–100.

    CAS  Article  Google Scholar 

  7. Lowe B, Venkatesan J, Anil S, Shim MS, Kim SK. Preparation and characterization of chitosan-natural nano hydroxyapatite-fucoidan nanocomposites for bone tissue engineering. Int J Biol Macromol. 2016;93:1479–87.

    CAS  Article  Google Scholar 

  8. Chakraborty R, Roy Chowdhury D. Fish bone derived natural hydroxyapatite-supported copper acid catalyst: taguchi optimization of semibatch oleic acid esterification. Chem Eng J. 2013;215:491–9.

    Article  Google Scholar 

  9. Brzezińska-Miecznik J, Haberko K, Sitarz M, Bućko MM, Macherzyńska B, Lach R. Natural and synthetic hydroxyapatite/zirconia composites: a comparative study. Ceram Int. 2016;42:11126–35.

    Article  Google Scholar 

  10. Giraldo-Betancur AL, Espinosa-Arbelaez DG, del Real-López A, Millan-Malo BM, Rivera-Muñoz EM, Gutierrez-Cortez E et al. Comparison of physicochemical properties of bio and commercial hydroxyapatite. Curr Appl Phys. 2013;13:1383–90.

    Article  Google Scholar 

  11. Lombardi M, Palmero P, Haberko K, Pyda W, Montanaro L. Processing of a natural hydroxyapatite powder: from powder optimization to porous bodies development. J Eur Ceram Soc. 2011;31:2513–8.

    CAS  Article  Google Scholar 

  12. Niakan A, Ramesh S, Ganesan P, Tan CY, Purbolaksono J, Chandran H et al. Sintering behaviour of natural porous hydroxyapatite derived from bovine bone. Ceram Int. 2015;41:3024–9.

    CAS  Article  Google Scholar 

  13. Swain SK, Bhattacharyya S. Preparation of high strength macroporous hydroxyapatite scaffold. Mater Sci Eng: C. 2013;33:67–71.

    CAS  Article  Google Scholar 

  14. Mondal S, Mondal B, Dey A, Mukhopadhyay SS. Studies on processing and characterization of hydroxyapatite biomaterials from different bio wastes. J Miner Mater Charact Eng. 2012;11:55.

    Google Scholar 

  15. Akram M, Ahmed R, Shakir I, Ibrahim WA, Hussain R. Extracting hydroxyapatite and its precursors from natural resources. J Mater Sci. 2014;49:1461–75.

    CAS  Article  Google Scholar 

  16. Ooi CY, Hamdi M, Ramesh S. Properties of hydroxyapatite produced by annealing of bovine bone. Ceram Int. 2007;33:1171–7.

    CAS  Article  Google Scholar 

  17. Younesi M, Javadpour S, Bahrololoom ME. Effect of heat treatment temperature on chemical compositions of extracted hydroxyapatite from bovine bone ash. J Mater Eng Perform. 2011;20:1484–90.

    CAS  Article  Google Scholar 

  18. Krzesińska M, Majewska J. Physical properties of continuous matrix of porous natural hydroxyapatite related to the pyrolysis temperature of animal bones precursors. J Anal Appl Pyrolysis. 2015;116:202–14.

    Article  Google Scholar 

  19. Hui P, Meena SL, Singh G, Agarawal RD, Prakash S. Synthesis of hydroxyapatite bio-ceramic powder by hydrothermal method. J Miner Mater Charact Eng. 2010;9:683.

    Google Scholar 

  20. Yeong KC, Wang J, Ng SC. Mechanochemical synthesis of nanocrystalline hydroxyapatite from CaO and CaHPO4. Biomaterials. 2001;22:2705–12.

    CAS  Article  Google Scholar 

  21. Tlotleng M, Akinlabi E, Shukla M, Pityana S. Microstructures, hardness and bioactivity of hydroxyapatite coatings deposited by direct laser melting process. Mater Sci Eng: C. 2014;43:189–98.

    CAS  Article  Google Scholar 

  22. Raya I, Mayasari E, Yahya A, Syahrul M, Latunra AI. Synthesis and characterizations of calcium hydroxyapatite derived from crabs shells (Portunus pelagicus) and its potency in safeguard against to dental demineralizations. Int J Biomater. 2015;469176:8.

    Google Scholar 

  23. Sunil BR, Jagannatham M. Producing hydroxyapatite from fish bones by heat treatment. Mater Lett. 2016;185:411–4.

    CAS  Article  Google Scholar 

  24. Mukherjee S, Nandi SK, Kundu B, Chanda A, Sen S, Das PK. Enhanced bone regeneration with carbon nanotube reinforced hydroxyapatite in animal model. J Mech Behav Biomed Mater. 2016;60:243–55.

    CAS  Article  Google Scholar 

  25. Zhao XY, Zhu YJ, Chen F, Lu BQ, Wu J. Nanosheet-assembled hierarchical nanostructures of hydroxyapatite: surfactant-free microwave-hydrothermal rapid synthesis, protein/DNA adsorption and pH-controlled release. CrystEngComm. 2013;15:206–12.

    CAS  Article  Google Scholar 

  26. Kusrini E, Sontang M. Characterization of x-ray diffraction and electron spin resonance: Effects of sintering time and temperature on bovine hydroxyapatite. Radiat Phys Chem. 2012;81:118–25.

    CAS  Article  Google Scholar 

  27. Haberko K, Bućko MM, Brzezińska-Miecznik J, Haberko M, Mozgawa W, Panz T et al. Natural hydroxyapatite—its behaviour during heat treatment. J Eur Ceram Soc. 2006;26:537–42.

    CAS  Article  Google Scholar 

  28. Murugan R, Ramakrishna S, Rao KP. Nanoporous hydroxy-carbonate apatite scaffold made of natural bone. Mater Lett. 2006;60:2844–7.

    CAS  Article  Google Scholar 

  29. Duan S, Feng P, Gao C, Xiao T, Yu K, Shuai C et al. Microstructure evolution and mechanical properties improvement in liquid-phase-sintered hydroxyapatite by laser sintering. Materials. 2015;8:1162–75.

    CAS  Article  Google Scholar 

  30. Radha G, Balakumar S, Venkatesan B, Vellaichamy E. Evaluation of hemocompatibility and in vitro immersion on microwave-assisted hydroxyapatite–alumina nanocomposites. Mater Sci Eng: C. 2015;50:143–50.

    CAS  Article  Google Scholar 

  31. Khoo W, Nor FM, Ardhyananta H, Kurniawan D. Preparation of natural hydroxyapatite from bovine femur bones using calcination at various temperatures. Procedia Manuf. 2015;2:196–201.

    Article  Google Scholar 

  32. Trinkūnaitė-Felsen J, Žalga A, Kareiva A. Characterization of naturally derived calcium compounds used in food industry. Chemija. 2012;23:76–85.

    Google Scholar 

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Affiliations

  1. Department of Ceramic Technology, Alagapa College of Technology, Anna University, Chennai, TN, 600 025, India

    S. Aarthy, D. Thenmuhil & P. Manohar

  2. Biomaterials Department, CSIR-Central Leather Research Institute, Adyar, Chennai, TN, 600 020, India

    G. Dharunya

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  1. S. Aarthy
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  2. D. Thenmuhil
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  3. G. Dharunya
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Correspondence to P. Manohar.

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Aarthy, S., Thenmuhil, D., Dharunya, G. et al. Exploring the effect of sintering temperature on naturally derived hydroxyapatite for bio-medical applications. J Mater Sci: Mater Med 30, 21 (2019). https://doi.org/10.1007/s10856-019-6219-9

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  • DOI: https://doi.org/10.1007/s10856-019-6219-9