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Human amniotic membrane for guided bone regeneration of calvarial defects in mice

  • Tissue Engineering Constructs and Cell Substrates
  • Original Research
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Abstract

Due to its biological properties, human amniotic membrane (hAM) is widely studied in the field of tissue engineering and regenerative medicine. hAM is already very attractive for wound healing and it may be helpful as a support for bone regeneration. However, few studies assessed its potential for guided bone regeneration (GBR). The purpose of the present study was to assess the potential of the hAM as a membrane for GBR. In vitro, cell viability in fresh and cryopreserved hAM was assessed. In vivo, we evaluated the impact of fresh versus cryopreserved hAM, using both the epithelial or the mesenchymal layer facing the defect, on bone regeneration in a critical calvarial bone defect in mice. Then, the efficacy of cryopreserved hAM associated with a bone substitute was compared to a collagen membrane currently used for GBR. In vitro, no statistical difference was observed between the conditions concerning cell viability. Without graft material, cryopreserved hAM induced more bone formation when the mesenchymal layer covered the defect compared to the defect left empty. When associated with a bone substitute, such improved bone repair was not observed. These preliminary results suggest that cryopreserved hAM has a limited potential for GBR.

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Abbreviations

BMP :

Bone morphogenetic protein

GBR :

Guided bone regeneration

HA :

Hydroxy apatite

hAM :

human amniotic membrane

hAECs :

human amniotic epithelial cells

hAMSCs :

human amniotic mesenchymal stem cells

f-hAM :

fresh hAM

Cryo-hAM :

Cryopreserved hAM

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Acknowledgements

La Fondation des gueules cassées for financial support. Ray Cooke (professional proof-reader) for copyediting the manuscript. Patrick Guitton for his contribution to design the figures.

Author contributions

MF (1st author): Contributed to conception and design of the experimental studies (both in vitro and in vivo studies); Contributed to data acquisition, analysis, and data interpretation; Drafted the manuscript; Critically revised the manuscript; Gave final approval; Agrees to be accountable for all aspects of work ensuring integrity and accuracy. OC(2nd author): X-ray analyzes; Gave final approval; Agrees to be accountable for all aspects of work ensuring integrity and accuracy. JK(3rd author): Obtained hydroxyapatite particles; Critically revised the manuscript; Gave final approval; Agrees to be accountable for all aspects of work ensuring integrity and accuracy. FG (4th author): Contributed to conception and design of the experimental in vivo studies; Critically revised the manuscript; Gave final approval; Agrees to be accountable for all aspects of work ensuring integrity and accuracy. SB (5th author): contributed to collection of human placentas; Critically revised the manuscript; Gave final appoval; Agrees to be accountable for all aspects of work ensuring integrity and accuracy RB (6th author): contributed to conception and design of in vitro studies; Critically revised the manuscript; Gave final appoval; Agrees to be accountable for all aspects of work ensuring integrity and accuracyZI (57th author): Contributed to collection of human placentas; Critically revised the manuscript; Gave final approval, Agrees to be accountable for all aspects of work ensuring integrity and accuracy. JCF (last co-author): Contributed to conception and design of experimental studies (both in vitro and in vivo studies); Contributed to analysis, and interpretation of the data; Drafted the manuscript; Critically revised the manuscript; Gave final approvalAgrees to be accountable for all aspects of work ensuring integrity and accuracy. CB (Last co- author); Contributed to conception and design of experimental studies (both in vitro and in vivo studies); Contributed to data acquisition, analysis, and data interpretation; Drafted the manuscript; Gave final approval; Agrees to be accountable for all aspects of work ensuring integrity and accuracy.

Funding

Fondation des gueules cassées.

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Authors and Affiliations

  1. Univ. Bordeaux, INSERM, Laboratory BioTis, UMR 1026, F-33076, Bordeaux, France

    Mathilde Fénelon, Olivier Chassande, Jérome Kalisky, Reine Bareille, Jean-Christophe Fricain & Claudine Boiziau

  2. CHU Bordeaux, Odontology and Oral Health Department, F-33076, Bordeaux, France

    Mathilde Fénelon & Jean-Christophe Fricain

  3. Orthopedic, Traumatologic & Plastic Surgery Service - University Hospital of Besançon, Besançon, France

    Florelle Gindraux

  4. University hospital, Gynecology-Obstetrics Service, F-33076, Bordeaux, France

    Stéphanie Brun

  5. French Blood Establishment (EFS), Aquitaine-Limousin Branch, Bordeaux, France

    Zoran Ivanovic

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  1. Mathilde Fénelon
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  2. Olivier Chassande
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  3. Jérome Kalisky
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  7. Zoran Ivanovic
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  8. Jean-Christophe Fricain
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  9. Claudine Boiziau
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Corresponding author

Correspondence to Mathilde Fénelon.

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The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Ethics approval

The present study was approved by the French Ethics Committee (agreement APAFIS n 2685-20l5111012075358 v4).

Additional information

Jean-Christophe Fricain and Claudine Boiziau are co-directed.

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Fénelon, M., Chassande, O., Kalisky, J. et al. Human amniotic membrane for guided bone regeneration of calvarial defects in mice. J Mater Sci: Mater Med 29, 78 (2018). https://doi.org/10.1007/s10856-018-6086-9

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