Abstract
In this study, 3D macroporous bioscaffolds were developed from poly(dimethylsiloxane) (PDMS) which is inert, biocompatible, non-biodegradable, retrievable and easily manufactured at low cost. PDMS bioscaffolds were synthesized using a solvent casting and particulate leaching (SCPL) technique and exhibited a macroporous interconnected architecture with 86 ± 3% porosity and 300 ± 100 µm pore size. As PDMS intrinsically has a hydrophobic surface, mainly due to the existence of methyl groups, its surface was modified by oxygen plasma treatment which, in turn, enabled us to apply a novel polydopamine coating onto the surface of the bioscaffold. The addition of a polydopamine coating to bioscaffolds was confirmed using composition analysis. Characterization of oxygen plasma treated-PDMS bioscaffolds coated with polydopamine (polydopamine coated-PDMS bioscaffolds) showed the presence of hydroxyl and secondary amines on their surface which resulted in a significant decrease in water contact angle when compared to uncoated-PDMS bioscaffolds (35 ± 3%, P < 0.05). Seeding adipose tissue-derived mesenchymal stem cells (AD-MSCs) into polydopamine coated-PDMS bioscaffolds resulted in cells demonstrating a 70 ± 6% increase in viability and 40 ± 5% increase in proliferation when compared to AD-MSCs seeded into uncoated-PDMS bioscaffolds (P < 0.05). In summary, this two-step method of oxygen plasma treatment followed by polydopamine coating improves the biocompatibility of PDMS bioscaffolds and only requires the use of simple reagents and mild reaction conditions. Hence, our novel polydopamine coated-PDMS bioscaffolds can represent an efficient and low-cost bioscaffold platform to support MSC therapies.
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References
Nolan K, Millet Y, Ricordi C, Stabler CL. Tissue engineering and biomaterials in regenerative medicine. Cell Transplant. 2008;17:241–243.
Danilov V, Dölle C, Ott M, Wagner H, Meichsner J. Plasma treatment of polydimethylsiloxane thin films studied by infrared reflection absorption spectroscopy. 29thICPIG, July 12-17, Cancún, México, https://pdfs.semanticscholar.org/dafa/f2d21dde70ccda35056a4f0113ae4aaaadda.pdf?_ga=2.94902553.463489516.1524765127-1439881367.1524765127. 2009.
Matsuda K, Suzuki S, Isshiki N, Ikada Y. Re-freeze dried bilayer artificial skin. Biomaterials. 1993;14:1030–5.
Kiremitçi M, Şerbetçi AI, Çolak R, Pişkin E. Cell attachment to PU and PHEMA based biomaterials: relation to structural properties. Clin Mater. 1991;8:9–16.
Khorasani MT, Mirzadeh H. BHK cells behaviour on laser treated polydimethylsiloxane surface. Colloids Surf B Biointerfaces. 2004;35:67–71.
Liu M, Chen Q. Characterization study of bonded and unbonded polydimethylsiloxane aimed for bio-micro-electromechanical systems-related applications. J Micro/Nanolithogr, MEMS, Moems. 2007;6:23008.
Khanafer K, Duprey A, Schlicht M, Berguer R. Effects of strain rate, mixing ratio, and stress-strain definition on the mechanical behavior of the polydimethylsiloxane (PDMS) material as related to its biological applications. Biomed Micro. 2009;11:503–8.
Colas A, Curtis J. Silicone biomaterials: history and chemistry & medical applications of silicones. Repr Biomater Sci. 2005;80–6:697–707.
Pedraza E, Brady AC, Fraker CA, Stabler CL. Synthesis of macroporous poly(dimethylsiloxane) scaffolds for tissue engineering applications. J Biomater Sci Ed. 2013;24:1041–56.
Hassler C, Boretius T, Stieglitz T. Polymers for neural implants. J Polym Sci Part B Polym Phys2011;49:18–33.
Si J,Cui Z,Xie P,Song L,Wang Q,Liu Q, et al. Characterization of 3D elastic porous polydimethylsiloxane (PDMS) cell scaffolds fabricated by VARTM and particle leaching. J Appl Polym Sci. 2016;42909:1–9.
Bodas D, Khan-Malek C. Hydrophilization and hydrophobic recovery of PDMS by oxygen plasma and chemical treatment-An SEM investigation. Sens Actuators, B Chem. 2007;123:368–73.
Halldorsson S, Lucumi E, Gómez-Sjöberg R, Fleming RMT. Advantages and challenges of microfluidic cell culture in polydimethylsiloxane devices. Biosens Bioelectron. 2015;63:218–31.
Lee JN, Jiang X, Ryan D, Whitesides GM. Compatibility of mammalian cells on surfaces of poly(dimethylsiloxane). Langmuir. 2004;20:11684–91.
Fuard D, Tzvetkova-Chevolleau T, Decossas S, Tracqui P, Schiavone P. Optimization of poly-di-methyl-siloxane (PDMS) substrates for studying cellular adhesion and motility. Microelectron Eng. 2008;85:1289–93.
Chuah YJ, Kuddannaya S, Lee MHA, Zhang Y, Kang Y. The effects of poly(dimethylsiloxane) surface silanization on the mesenchymal stem cell fate. Biomater Sci. 2015;3:383–90.
Chuah YJ, Koh YT, Lim K, Menon NV, Wu Y, Kang Y. Simple surface engineering of polydimethylsiloxane with polydopamine for stabilized mesenchymal stem cell adhesion and multipotency. Sci Rep. 2015;5:18162.
Ye Q, Zhou F, Liu W. Bioinspired catecholic chemistry for surface modification. Chem Soc Rev. 2011;40:4244.
Kuddannaya S, Chuah Y. Surface chemical modification of poly (dimethylsiloxane) for the enhanced adhesion and proliferation of mesenchymal stem cells. ACS Appl Mater Interfaces. 2013;5:9777–84.
Huang S, Liang N, Hu Y, Zhou X, Abidi N. Polydopamine-assisted surface modification for bone biosubstitutes. Biomed Res. Int. 2016;2016:1–9.
Lee H, Dellatore SM, Miller WM, Messersmith PB. Mussel-inspired surface chemistry for multifunctional coatings. Science. 2007;318:426–30.
Tan SH, Nguyen NT, Chua YC, Kang TG. Oxygen plasma treatment for reducing hydrophobicity of a sealed polydimethylsiloxane microchannel. Biomicrofluidics. 2010;4:32204.
Hillborg H, Ankner JF, Gedde UW, Smith GD, Yasuda HK, Wikström K. Crosslinked polydimethylsiloxane exposed to oxygen plasma studied by neutron reflectometry and other surface specific techniques. Polymer (Guildf). 2000;41:6851–63.
Bhat S, Kumar A. Biomaterials in regenerative medicine. J Post Med Edu Res. 2012;46:81–9.
Caplan AI. Adult mesenchymal stem cells for tissue engineering versus regenerative medicine. J Cell Physiol. 2007;213:341–7.
Serup P, Madsen OD, Mandrup-Poulsen T. Islet and stem cell transplantation for treating diabetes. BMJ. 2001;322:29–32.
Krishnan R, Alexander M, Robles L, Foster CE, Lakey JRT. Islet and stem cell encapsulation for clinical transplantation. Rev Diabet Stud. 2014;11:84–101.
Ito T, Itakura S, Todorov I, Rawson J, Asari S, Shintaku J, et al. Mesenchymal stem cell and islet co-transplantation promotes graft revascularization and function. Transplantation. 2010;89:1438–45.
Yang F, Zhao B. Adhesion properties of self-polymerized dopamine thin film. Open Surf Sci J. 2011;3:115–22.
Tsai WB, Chen WT, Chien HW, Kuo WH, Wang MJ. Poly(dopamine) coating of scaffolds for articular cartilage tissue engineering. Acta Biomater. 2011;7:4187–94.
Ko E, Yang K, Shin J, Cho SW. Polydopamine-assisted osteoinductive peptide immobilization of polymer scaffolds for enhanced bone regeneration by human adipose-derived stem cells. Biomacromolecules. 2013;14:3202–13.
Tsai WB, Chen WT, Chien HW, Kuo WH, Wang MJ. Poly(dopamine) coating to biodegradable polymers for bone tissue engineering. J Biomater Appl. 2014;28:837–48.
Pariente J-L, Kim B-S, Atala A. In vitro biocompatibility evaluation of naturally derived and synthetic biomaterials using normal human bladder smooth muscle cells. J Urol. 2002;167:1867–71.
Song E, Yeon Kim S, Chun T, Byun HJ, Lee YM. Collagen scaffolds derived from a marine source and their biocompatibility. Biomaterials. 2006;27:2951–61.
Duffy DC, McDonald JC, Schueller OJA, Whitesides GM. Rapid prototyping of microfluidic systems in poly(dimethylsiloxane). Anal Chem. 1998;70:4974–84.
McDonald JC, Whitesides GM. Poly(dimethylsiloxane) as a material for fabricating microfluidic devices. Acc Chem Res. 2002;35:491–9.
Mata A, Fleischman AJ, Roy S. Characterization of polydimethylsiloxane (PDMS) properties for biomedical micro/nanosystems. Biomed Micro. 2005;7:281–93.
Kumari J, Karande AA, Kumar A. Combined effect of cryogel matrix and temperature-reversible soluble-insoluble polymer for the development of in vitro human liver tissue. ACS Appl Mater Interfaces. 2016;8:264–77.
Matsiko A, Gleeson JP, O’Brien FJ. Scaffold mean pore size influences mesenchymal stem cell chondrogenic differentiation and matrix deposition. Tissue Eng Part A. 2015;21:486–97.
Bencherif SA, Warren Sands R, Ali OA, Li WA, Lewin SA, Braschler TM, et al. Injectable cryogel-based whole-cell cancer vaccines. Nat Commun. 2015;6:7556.
Mandal BB, Kundu SC. Cell proliferation and migration in silk fibroin 3D scaffolds. Biomaterials. 2009;30:2956–65.
Kumari J,Kumar A, Development of polymer based cryogel matrix for transportation and storage of mammalian cells. Sci Rep [Internet]. 2017;7:41551.
Yoo HS, Kim TG, Park TG. Surface-functionalized electrospun nanofibers for tissue engineering and drug delivery. Adv Drug Deliv Rev. 2009;61:1033–42.
Martins A, Pinho ED, Faria S, Pashkuleva I, Marques AP, Reis RL, et al. Surface modification of electrospun polycaprolactone nanofiber meshes by plasma treatment to enhance biological performance. Small. 2009;5:1195–206.
Ma Z, Gao C, Gong Y, Shen J. Cartilage tissue engineering PLLA scaffold with surface immobilized collagen and basic fibroblast growth factor. Biomaterials. 2005;26:1253–9.
Yu H, Chong ZZ, Tor SB, Liu E, Loh NH. Low temperature and deformation-free bonding of PMMA microfluidic devices with stable hydrophilicity via oxygen plasma treatment and PVA coating. RSC Adv. 2015;5:8377–88.
Hui AYN, Wang G, Lin B, Chan W-T. Microwave plasma treatment of polymer surface for irreversible sealing of microfluidic devices. Lab Chip. 2005;5:1173–7.
Tsougeni K, Vourdas N, Tserepi A, Gogolides E, Cardinaud C. Mechanisms of oxygen plasma nanotexturing of organic polymer surfaces: from stable super hydrophilic to super hydrophobic surfaces. Langmuir. 2009;25:11748–59.
Bhattacharya S, Datta A, Berg JM, Gangopadhyay S. Studies on surface wettability of poly(dimethyl) siloxane (PDMS) and glass under oxygen-plasma treatment and correlation with bond strength. J Micro Syst. 2005;14:590–7.
Morra M, Occhiello E, Marola R, Garbassi F, Humphrey P, Johnson D. On the aging of oxygen plasma-treated polydimethylsiloxane surfaces. J Colloid Interface Sci. 1990;137:11–24.
Tóth A, Bertóti I, Blazsó M, Bánhegyi G, Bognar A, Szaplonczay P. Oxidative damage and recovery of silicone rubber surfaces. I. X‐ray photoelectron spectroscopic study. J Appl Polym Sci. 1994;52:1293–307.
Eddington DT, Puccinelli JP, Beebe DJ. Thermal aging and reduced hydrophobic recovery of polydimethylsiloxane. Sens Actuators, B Chem. 2006;114:170–2.
Kim J, Chaudhury MK, Owen MJ, Orbeck T. The mechanisms of hydrophobic recovery of polydimethylsiloxane elastomers exposed to partial electrical discharges. J Colloid Interface Sci. 2001;244:200–7.
Chen IJ, Lindner E. The stability of radio-frequency plasma-treated polydimethylsiloxane surfaces. Langmuir. 2007;23:3118–22.
Hashimoto M, Shevkoplyas SS, Zasońska B, Szymborski T, Garstecki P, Whitesides GM. Formation of bubbles and droplets in parallel, coupled flow-focusing geometries. Small. 2008;4:1795–805.
Roth J, Albrecht V, Nitschke M, Bellmann C, Simons F, Zschoche S, et al. Surface functionalization of silicone rubber for permanent adhesion improvement. Langmuir. 2008;24:12603–11.
Matějíček J, Vilémová M, Mušálek R, Sachr P, Horník J. The influence of interface characteristics on the adhesion/cohesion of plasma sprayed tungsten coatings. Coatings. 2013;3:108–25.
Fu J,Chuah YJ,Ang WT,Zheng N,Wang D-A, Optimization of a polydopamine (PD)-based coating method and polydimethylsiloxane (PDMS) substrates for improved mouse embryonic stem cell (ESC) pluripotency maintenance and cardiac differentiation. Biomater Sci. 2017;5:1156–73. http://xlink.rsc.org/?DOI=C7BM00266A.
Rim NG, Kim SJ, Shin YM, Jun I, Lim DW, Park JH, et al. Mussel-inspired surface modification of poly(l-lactide) electrospun fibers for modulation of osteogenic differentiation of human mesenchymal stem cells. Colloids Surf B-Biointerfaces. 2012;91:189–97.
Gomathi N,Mishra I,Varma S,Neogi S., Surface modification of poly(dimethylsiloxane) through oxygen and nitrogen plasma treatment to improve its characteristics towards biomedical applications. Surf Topogr Metrol Prop. 2015;035005:1–14.
Ding YH, Floren M, Tan W. Mussel-inspired polydopamine for bio-surface functionalization. Biosurf Biotribol. 2016;2:121–36.
Lee H, Dellatore SM, Miller WM, Messersmith PB. Mussel-inspired surface chemistry for multifunctional coatings. Science. 2007;318:426–30.
Hong S, Kim KY, Wook HJ, Park SY, Lee KD, Lee DY, et al. Attenuation of the in vivo toxicity of biomaterials by polydopamine surface modification. Nanomedicine. 2011;6:793–801.
Jiang X, Christopherson GT, Mao H-Q. The effect of nanofibre surface amine density and conjugate structure on the adhesion and proliferation of human haematopoietic progenitor cells. Interface Focus. 2011;1:725–33.
Lee JH, Jung HW, Kang I-K, Lee HB. Cell behaviour on polymer surfaces with different functional groups. Biomaterials. 1994;15:705–11.
Shin YM, Lee YB, Kim SJ, Kang JK, Park JC, Jang W, et al. Mussel-inspired immobilization of vascular endothelial growth factor (VEGF) for enhanced endothelialization of vascular grafts. Biomacromolecules. 2012;13:2020–8.
Acknowledgements
This work was supported by Stanford Nano Shared Facilities (SNSF) grant (1161726-146-DAARZ), as part of the grant supported by the National Science Foundation grant (ECCS-1542152) and the Stanford Neuroscience Microscopy Service grant (NIH NS069375).
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Razavi, M., Thakor, A.S. An oxygen plasma treated poly(dimethylsiloxane) bioscaffold coated with polydopamine for stem cell therapy. J Mater Sci: Mater Med 29, 54 (2018). https://doi.org/10.1007/s10856-018-6077-x
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DOI: https://doi.org/10.1007/s10856-018-6077-x