Biocompatibility of new materials based on nano-structured nitinol with titanium and tantalum composite surface layers: experimental analysis in vitro and in vivo

  • Mikhail A. Sevost’yanov
  • Elena O. Nasakina
  • Alexander S. Baikin
  • Konstantin V. Sergienko
  • Sergey V. Konushkin
  • Mikhail A. Kaplan
  • Alexey V. Seregin
  • Alexander V. Leonov
  • Valery A. Kozlov
  • Alexey V. Shkirin
  • Nikolai F. Bunkin
  • Alexey G. Kolmakov
  • Sergey V. Simakov
  • Sergey V. Gudkov
Biocompatibility Studies Original Research
  • 111 Downloads
Part of the following topical collections:
  1. Biocompatibility Studies

Abstract

A technology for obtaining materials from nanostructured nitinol with titanium- or tantalum-enriched surface layers was developed. Surface layers enriched with titanium or tantalum were shown to provide a decrease in the formation of reactive oxygen species and long-lived protein radicals in comparison to untreated nitinol. It was determined that human peripheral vessel myofibroblasts and human bone marrow mesenchymal stromal cells grown on nitinol bases coated with titanium or tantalum-enriched surface layers exhibit a nearly two times higher mitotic index. Response to implantation of pure nitinol, as well as nano-structure nitinol with titanium or tantalum-enriched surface layers, was expressed though formation of a mature uniform fibrous capsule peripherally to the fragment. The thickness of this capsule in the group of animals subjected to implantation of pure nitinol was 1.5 and 3.0-fold greater than that of the capsule in the groups implanted with nitinol fragments with titanium- or tantalum-enriched layers. No signs of calcinosis in the tissues surrounding implants with coatings were observed. The nature and structure of the formed capsules testify bioinertia of the implanted samples. It was shown that the morphology and composition of the surface of metal samples does not alter following biological tests. The obtained results indicate that nano-structure nitinol with titanium or tantalum enriched surface layers is a biocompatible material potentially suitable for medical applications.

Notes

Acknowledgements

The authors are would like to thank Ph.D. Fadeev R.S. for helping with cell cultures and Ph.D. Fadeeva I.S. for help with animal treatment. The study was conducted with the support of a RFFR grant “14-29-10208-ofi_m” and the Russian President Program for Young Scientists “MK-4521.2018.8”. The work on optical microscopy is supported by the research topic “AAAA-A18-118021390190-1”.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Mikhail A. Sevost’yanov
    • 1
  • Elena O. Nasakina
    • 1
  • Alexander S. Baikin
    • 1
  • Konstantin V. Sergienko
    • 1
  • Sergey V. Konushkin
    • 1
  • Mikhail A. Kaplan
    • 1
  • Alexey V. Seregin
    • 1
    • 2
  • Alexander V. Leonov
    • 1
    • 2
  • Valery A. Kozlov
    • 2
    • 3
  • Alexey V. Shkirin
    • 3
    • 4
  • Nikolai F. Bunkin
    • 2
    • 3
  • Alexey G. Kolmakov
    • 1
  • Sergey V. Simakov
    • 1
  • Sergey V. Gudkov
    • 3
    • 5
    • 6
  1. 1.Baikov Institute of Metallurgy and Materials ScienceRussian Academy of SciencesMoscowRussia
  2. 2.Bauman Moscow State Technical UniversityMoscowRussia
  3. 3.A.M. Prokhorov General Physics InstituteRussian Academy of SciencesMoscowRussia
  4. 4.National Research Nuclear University MEPhIMoscowRussia
  5. 5.Depatment of BiophysicsLobachevsky State University of Nizhni NovgorodNizhni NovgorodRussia
  6. 6.Moscow Regional Research and Clinical Institute (MONIKI)MoscowRussia

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