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Repositing honey incorporated electrospun nanofiber membranes to provide anti-oxidant, anti-bacterial and anti-inflammatory microenvironment for wound regeneration

  • Ripon Sarkar
  • Aritri Ghosh
  • Ananya Barui
  • Pallab Datta
Tissue Engineering Constructs and Cell Substrates Original Research
Part of the following topical collections:
  1. Tissue Engineering Constructs and Cell Substrates

Abstract

Topical application of honey for tissue regeneration, has recently regained attention in clinical practice with controlled studies affirming its efficacy and indicating its role in regeneration over repair. Parallely, to overcome difficulties of applying raw honey, several product development studies like nanofibrous matrices have been reported. However, one approach concentrated on achieving highest possible honey loading in the nanofiber membranes while other studies have found that only specific honey dilutions result in differential cellular responses on wound healing and re-epithelization. From these results, it can be suggested that high honey loading provides optimum external microenvironment, low-loaded membranes could provide a more conducive internal microenvironment for tissue regeneration. With this hypothesis, this paper sought to evaluate ability of low-honey loaded nanofibers to modulate the anti-oxidant, anti-biofilm and anti-inflammatory properties which are important to be maintained in wound micro-environment. A loading-dependent reduction of biofilm formation and anti-oxidant activity was noted in different concentration ranges investigated. After scratch assay, a certain honey loading (0.5%) afforded the maximum re-epithelization. Since there is lack of methods to determine anti-inflammatory properties of nanofiber membranes during epithelial healing process, we performed anti-inflammatory assessment of nano-fibers by evaluating the expressions of pro-inflammatory markers-Cycloxygenase-2 (COX-2) and Interleukin-6 (IL-6) and to confirm the optimized concentration. Considering the role of COX-2 and IL-6, the novel methodology used in this study can also be developed as an assay for anti-inflammatory matrices for wound healing.

List of Abbreviation

COX-2

Cycloxygenase-2

IL6

Interleukin-6

PNL

Polymorphonuclear luekocyte

MMPs

Metalloproteinases

PDGF

Platelet derived growth factor

VEGF

Vascular endothelial growth factor

TGF-β

Transforming growth factor

ECM

Extra cellular matrix

MTT

3-[4,5-dimethylthiazol-2-yl]-diphenyltetrazolium bromide

BrdU

Bromodeoxyuridine

FBS

Fetal bovine serum

PVA

Poly vinyl alcohol

SEM

Scanning Electron Microscopy

MTT

3-[4,5-dimethylthiazol-2-yl]-diphenyltetrazolium bromide

BrdU

Bromodeoxyuridine

FBS

fetal bovine serum

PVA

Poly vinyl alcohol

SEM

Scanning Electron Microscopy

DMEM

Dulbecco’s modified Eagle’s medium

DIC

Differential interference contrast

PH

PVA-honey

Notes

Acknowledgements

AB would like to acknowledge SERB, Govt. of India financial assistance through Fast Track project numbers SB/FTP/ETA/265-2012 and DST INSPIRE faculty award to PD via IFA2012-LSBM-48. Assistance from TEQIP Phase II to IIEST Shibpur for procurement of Inverted Florescence microscope and TEQIP COE for AFM is also acknowledged.

Compliance with ethical standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

10856_2018_6038_MOESM1_ESM.docx (397 kb)
Supplementary Information(DOCX 397 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Ripon Sarkar
    • 1
  • Aritri Ghosh
    • 1
  • Ananya Barui
    • 1
  • Pallab Datta
    • 1
  1. 1.Centre for Healthcare Science and TechnologyIndian Institute of Engineering Science and Technology ShibpurHowrahIndia

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