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Alginate membranes loaded with hyaluronic acid and silver nanoparticles to foster tissue healing and to control bacterial contamination of non-healing wounds

  • Engineering and Nano-engineering Approaches for Medical Devices
  • Original Research
  • Published:
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Abstract

Chronic non-healing wounds are a clinically important problem in terms of number of patients and costs. Wound dressings such as hydrogels, hydrocolloids, polyurethane films and foams are commonly used to manage these wounds since they tend to maintain a moist environment which is shown to accelerate re-epithelialization. The use of antibacterial compounds is important in the management of wound infections. A novel wound-dressing material based on a blended matrix of the polysaccharides alginate, hyaluronic acid and Chitlac-silver nanoparticles is here proposed and its application for wound healing is examined. The manufacturing approach to obtain membranes is based on gelling, foaming and freeze-casting of alginate, hyaluronic acid and Chitlac-silver nanoparticles mixtures using calcium ions as the cross-linking agent. Comprehensive evaluations of the morphology, swelling kinetics, permeability, mechanical characteristics, cytotoxicity, capability to inhibit metalloproteinases and of antibacterial property were conducted. Biological in vitro studies demonstrated that hyaluronic acid released by the membrane is able to stimulate the wound healing meanwhile the metal silver exploits an efficient antibacterial activity against both planktonic bacteria and biofilms. Overall, the experimental data evidence that the studied material could be used as antibacterial wound dressing for wound healing promotion.

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Acknowledgements

The authors would like to acknowledge Dr. Gianluca Turco for providing assistance in SEM analysis and Dr. Matteo Crosera for ETAAS analysis.

Funding:

This study was supported by the EU-FP7 Project “AnastomoSEAL” (Contract Number 280929).

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Authors and Affiliations

  1. Department of Life Sciences, University of Trieste, Via Licio Giorgieri 5, I-34127, Trieste, Italy

    Lorena Tarusha, Sergio Paoletti & Andrea Travan

  2. Department of Medical, Surgical and Health Sciences, University of Trieste, Piazza dell’Ospitale 1, I-34129, Trieste, Italy

    Eleonora Marsich

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  1. Lorena Tarusha
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Correspondence to Eleonora Marsich.

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Tarusha, L., Paoletti, S., Travan, A. et al. Alginate membranes loaded with hyaluronic acid and silver nanoparticles to foster tissue healing and to control bacterial contamination of non-healing wounds. J Mater Sci: Mater Med 29, 22 (2018). https://doi.org/10.1007/s10856-018-6027-7

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  • DOI: https://doi.org/10.1007/s10856-018-6027-7

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