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A novel glucosamine derivative with low cytotoxicity enhances chondrogenic differentiation of ATDC5

  • Tissue Engineering Constructs and Cell Substrates
  • Original Research
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Abstract

Glucosamine (GlcN) is a component of native cartilage extracellular matrix and useful in cartilage repair, but it was limited by toxicity in high concentrations. With the aim of altering bioactive properties of GlcN to reduce the toxicity and to facilitate chondrogenesis for hyaline cartilage formation, we introduced an amino-group modification with double bond into GlcN to produce N-acryloyl-glucosamine (AGA). The cell ATDC5 was chosen to evaluate its cytotoxicity and chondrogenesis capability. Cell proliferation and cytotoxicity assay showed that AGA had significantly reduced the cytotoxicity compared to GlcN, and promoted ATDC5 proliferation. Alcian blue staining and biochemical analysis indicated that AGA enhanced extracellular matrix deposition. Both the mRNA and protein levels of articular cartilage markers, like Collagen II and Aggrecan were up-regulated, as shown by quantitative real-time PCR and immunofluorescence staining. Moreover, the level of fibrocartilage marker Collagen I and hypertrophic marker Collagen Χ weren’t significantly changed. Overall, these results demonstrated that the AGA achieved the functional double-bond, reduction in toxicity and enhancement in chondrogenesis could be more potential in cartilage repair.

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Acknowledgements

The work was supported in the part by the National Natural Science Foundation of China (51273072, 51673071, 51603073), Natural Science Foundation of Guangdong Province (2016A030313509), Pearl River S&T Nova Program of Guangzhou (201710010195), Guangdong Scientific and Technological Project (2014B090907004) and Guangzhou Important Scientific and Technological Special Project (201508020123). Dr. Wang acknowledges financial support from Ministry of Education Tier 1 Academic Research Fund (RG30/15 to Dong-An Wang). The authors thank the grant project of South China University of Technology for PhD student short term study abroad (outside mainland).

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Authors and Affiliations

  1. School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510041, China

    Hang Yao, Jingchen Xue, Renjian Xie, Sa Liu, Yingjun Wang, Wenjing Song & Li Ren

  2. National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou, 510006, China

    Hang Yao, Jingchen Xue, Renjian Xie, Sa Liu, Yingjun Wang, Wenjing Song & Li Ren

  3. Division of Bioengineering, School of Chemical & Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, N1.3-B2-13, Singapore, 637457, Singapore

    Hang Yao & Dong-An Wang

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  1. Hang Yao
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Correspondence to Wenjing Song, Dong-An Wang or Li Ren.

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Yao, H., Xue, J., Xie, R. et al. A novel glucosamine derivative with low cytotoxicity enhances chondrogenic differentiation of ATDC5. J Mater Sci: Mater Med 28, 170 (2017). https://doi.org/10.1007/s10856-017-5971-y

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