Antimicrobial and anti-biofilm properties of polypropylene meshes coated with metal-containing DLC thin films
- 189 Downloads
A promising strategy to reduce nosocomial infections related to prosthetic meshes is the prevention of microbial colonization. To this aim, prosthetic meshes coated with antimicrobial thin films are proposed. Commercial polypropylene meshes were coated with metal-containing diamond-like carbon (Me-DLC) thin films by the magnetron sputtering technique. Several dissimilar metals (silver, cobalt, indium, tungsten, tin, aluminum, chromium, zinc, manganese, tantalum, and titanium) were tested and compositional analyses of each Me-DLC were performed by Rutherford backscattering spectrometry. Antimicrobial activities of the films against five microbial species (Candida albicans, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Enterococcus faecalis) were also investigated by a modified Kirby-Bauer test. Results showed that films containing silver and cobalt have inhibited the growth of all microbial species. Tungsten-DLC, tin-DLC, aluminum-DLC, zinc-DLC, manganese-DLC, and tantalum-DLC inhibited the growth of some strains, while chromium- and titanium-DLC weakly inhibited the growth of only one tested strain. In-DLC film showed no antimicrobial activity. The effects of tungsten-DLC and cobalt-DLC on Pseudomonas aeruginosa biofilm formation were also assessed. Tungsten-DLC was able to significantly reduce biofilm formation. Overall, the experimental results in the present study have shown new approaches to coating polymeric biomaterials aiming antimicrobial effect.
The authors are grateful to the Laboratory of Analysis of Materials by Ionic Beams of the Institute of Physics-University of São Paulo for RBS analysis, Center for Radiation Technology of the Nuclear and Energy Research Institute for the sterilization of the samples, and the agencies Coordination for the Improvement of Higher Education Personnel and National Council for Scientific and Technological Development for the financial support.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no competing interests.
- 21.Hudzicki J. Kirby-Bauer Disk Diffusion Susceptibility Test Protocol. ASM MicrobeLibrary. 2013. http://www.microbelibrary.org/component/resource/laboratory-test/3189-kirby-bauer-disk-diffusion-susceptibility-test-protocol. Accessed 15 Sep 2016.
- 26.Rogers HJ, Woods VE, Synge C. Antibacterial effect of the Scandium and Indium complexes of enterochelin on Escherichia coli. J Gen Microbiol. 1982;128:2389–94.Google Scholar
- 33.Schiffmann KI, Fryda M, Goerigk G, Lauer R, Hinze P, Bulack A. Sizes and distances of metal clusters in Au-, Pt-, W- and Fe-containing diamond-like carbon hard coatings: a comparative study by small angle X-ray scattering, wide angle X-ray diffraction, transmission electron microscopy and scanning tunnelling microscopy. Thin Solid Films. 1999;347:60–71.CrossRefGoogle Scholar
- 36.Yuoh ACB, Agwara MO, Yufanyi DM, Conde MA, Jagan R, Eyong KO. Synthesis, crystal structure, and antimicrobial properties of a novel 1-D cobalt coordination polymer with dicyanamide and 2-aminopyridine. Int J Inorg Chem. 2015;2015:106838. http://dx.doi.org/10.1155/2015/106838.Google Scholar
- 45.Paéz PL, Bazán CM, Bongiovanni ME et al. Oxidative stress and antimicrobial activity of Chromium(III) and Ruthenium(II) complexes on Staphylococcus aureus and Escherichia coli. Biomed Res Int. 2013;2013.Google Scholar
- 48.Singh K, Kumar Y, Puri P, Sharma C, Aneja KR. Antimicrobial, spectral and thermal studies of divalent cobalt, nickel, copper and zinc complexes with triazole Schiff bases. Arab J Chem. 2013;10:S978–S987.Google Scholar
- 51.Chen Q, Thouas G. Biomaterials: a basic introduction. 1st ed. Boca Raton (FL): CRC Press; 2015.Google Scholar