Abstract
The aim of this study is to develop a simple, convenient and effective approach to synthesize nano-sized hydroxyapatite (nano-HA) at high-scale yield. Nano-HA was wet synthesized in the presence or absence of alendronate sodium (ALN), one of bisphosphonates for anti-osteoporotic. Then aged and washed nano-HA precipitate was directly treated by mechanical activation combined with the chemical dispersion of ALN to prevent the agglomeration of nano-HA. ALN acted not only as a chemical dispersant but also as an orthopedic drug. In vitro release showed that ALN was released slowly from nano-HA. Transmission electron microscopy (TEM) revealed that nano-HA with size less than 100 nm appeared as single particle after being treated by mechanical activation combined with the dispersion of ALN (AMA-HA and MA-HA). High resolution transmission electron microscopy (HRTEM) and X-ray diffraction (XRD) confirmed that as-prepared nanoparticles were HA with low crystallinity and crystallite size. Fourier transform infrared spectroscopy (FTIR) indicated that the phosphonate groups in ALN were introduced to bond with the Ca2+ of HA to impede the growth of HA crystal. Zeta potential illustrated that the absolute value of surface negative charge of nano-HA increased significantly with the addition of ALN, which inhibited the agglomeration of nano-HA. The present approach makes it feasible to produce nano-HA at high-scale yield, which provide the possibility to construct bone graft.
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Landi E, Tampieri A, Celotti G, Sprio S, Sandri M, Logroscino G. Sr-substituted hydroxyapatites for osteoporotic bone replacement. Acta Biomater. 2007;3:961–9.
Jarcho M. Calcium phosphate ceramics as hard tissue prosthetics. Clin Orthop Relat Res. 1981;157:259–78.
Krisanapiboon A, Buranapanitkit B, Oungbho K. Biocompatability of hydroxyapatite composite as a local drug delivery system. J Orthop Surg Res. 2006;14:315
Yao J, Tjandra W, Chen YZ, Tam KC, Ma J, Soh B. Hydroxyapatite nanostructure material derived using cationic surfactant as a template. J Mater Chem. 2003;13:3053–7.
Boonsongrit Y, Abe H, Sato K, Naito M, Yoshimura M, Ichikawa H, Fukumori Y. Controlled release of bovine serum albumin from hydroxyapatite microspheres for protein delivery system. Mater Sci Eng: B. 2008;148:162–5.
Zhu SH, Huang BY, Zhou KC, Huang SP, Liu F, Li YM, Long ZG. Hydroxyapatite nanoparticles as a novel gene carrier. J Nanopart Res. 2004;6:307–11.
Zhou H, Lee J. Nanoscale hydroxyapatite particles for bone tissue engineering. Acta Biomater. 2011;7:2769–81.
Garai S, Sinha A. Three dimensional biphasic calcium phosphate nanocomposites for load bearing bioactive bone grafts. Mater Sci Eng: C. 2016;59:375–83.
Boanini E, Torricelli P, Gazzano M, Giardino R, Bigi A. Alendronate-hydroxyapatite nanocomposites and their interaction with osteoclasts and osteoblast-like cells. Biomaterials. 2008;29:790–6.
Kumar AR, Kalainathan S. Sol–gel synthesis of nanostructured hydroxyapatite powder in presence of polyethylene glycol. Phys B Cond Matter. 2010;405:2799–802.
Catros S, Guillemot F, Lebraud E, Chanseau C, Perez S, Bareille R, Fricain JC. Physico-chemical and biological properties of a nano-hydroxyapatite powder synthesized at room temperature. Irbm. 2010;31:226–33.
Park HC, Baek DJ, Park YM, Yoon SY, Stevens R. Thermal stability of hydroxyapatite whiskers derived from the hydrolysis of α-TCP. J Mater Sci. 2004;39:2531–4.
Zhang G, Chen J, Yang S, Yu Q, Wang Z, Zhang Q. Preparation of amino-acid-regulated hydroxyapatite particles by hydrothermal method. Mater Lett. 2011;65:572–4.
Wei K, Wang Y, Lai C, Ning C, Wu D, Wu G, Ye J. Synthesis and characterization of hydroxyapatite nanobelts and nanoparticles. Mater Lett. 2005;59:220–5.
Silva CC, Graça MPF, Valente MA, Sombra ASB. Crystallite size study of nanocrystalline hydroxyapatite and ceramic system with titanium oxide obtained by dry ball milling. J Mater Sci. 2007;42:3851–5.
Fang W, Zhang H, Yin J, Yang B, Zhang Y, Li J, Yao F. Hydroxyapatite Crystal Formation in the Presence of Polysaccharide. Cryst Growth Des. 2016;16:1247–55.
Kakiage M, Iwase K, Kobayashi H. Effect of citric acid addition on disaggregation of crystalline hydroxyapatite nanoparticles under calcium-rich conditions. Mater Lett. 2015;156:39–41.
Deng ZY, Zhou Y, Inagaki Y, Ando M, Ohji T. Role of Zr(OH)4 hard agglomerates in fabricating porous ZrO2 ceramics and the reinforcing mechanism. Acta Mater. 2003;51:731–9.
Li H, Fu Y, Niu R, Zhou Z, Nie J, Yang D. Study on the biocomposites with poly (ethylene glycol) dimethacrylate and surfaced-grafted hydroxyapatite nanoparticles. J Appl Polym Sci. 2013;127:1737–43.
Zhang Y, Dong Y. Effect of surfactant on morphology of hydroxyapatite. Synth React Inorg M. 2015;45:411–4.
Gourion-Arsiquaud S, Allen MR, Burr DB, Vashishth D, Tang SY, Boskey AL. Bisphosphonate treatment modifies canine bone mineral and matrix properties and their heterogeneity. Bone. 2010;46:666–72.
Francis MD, Graham R, Russell G, Fleisch H. Diphosphonates inhibit formation of calcium phosphate crystals in vitro and pathological calcification in vivo. Science. 1969;165:1264–6.
Li DD, Zhu YT, Liang ZQ. Alendronate functionalized mesoporous hydroxyapatite nanoparticles for drug delivery. Mater Res Bull. 2013;48:2201–4.
Beyer MK, Clausen-Schaumann H. Mechanochemistry: the mechanical activation of covalent bonds. Chem Rev. 2005;105:2921–48.
Webster TJ, Siegel RW, Bizios R. Osteoblast adhesion on nanophase ceramics. Biomaterials. 1999;20:1221–7.
Silva CC, Pinheiro AG, Miranda MAR, Góes JC, Sombra ASB. Structural properties of hydroxyapatite obtained by mechanosynthesis. Solid State Sci. 2003;5:553–8.
Yeong KCB, Wang J, Ng SC. Mechanochemical synthesis of nanocrystalline hydroxyapatite from CaO and CaHPO4. Biomaterials. 2001;22:2705–12.
Nasiri-Tabrizi B, Fahami A, Ebrahimi-Kahrizsangi R. Effect of milling parameters on the formation of nanocrystalline hydroxyapatite using different raw materials. Ceram Int. 2013;39:5751–63.
Lopez-Heredia MA, Bohner M, Zhou W, Winnubst AJA, Wolke JGC, Jansen JA. The effect of ball milling grinding pathways on the bulk and reactivity properties of calcium phosphate cements. J Biomed Mater Res B. 2011;98:68–79.
Huang P, Li P, Zhao JS, Qu SX, Feng B, Weng J. Mechanical activation reinforced porous calcium phosphate cement. J Inorg Mater. 2015;30:432–8.
Mulak W, Balaž P, Chojnacka M. Chemical and morphological changes of millerite by mechanical activation. Int J Miner Process. 2002;66:233–40.
Mohandes F, Salavati-Niasari M. Simple morphology-controlled fabrication of hydroxyapatite nanostructures with the aid of new organic modifiers. Chem Eng J. 2014;252:173–84.
Taha EA, Youssef NF. Spectrophotometric determination of some drugs for osteoporosis. Chem Pharm Bull. 2003;51:1444–7.
Wang YJ, Chen JD, Wei K, Zhang SH, Wang XD. Surfactant-assisted synthesis of hydroxyapatite particles. Mater Lett. 2006;60:3227–31.
Habraken WJEM, Tao JH, Brylka LJ, Friedrich H, Bertinetti L, Schenk AS, Verch A, Dmitrovic V, Bomans PHH, Frederik PM, Laven J, Schoot P, Aichmayer B, With G, DeYoreo JJ, Sommerdijk NAJM. Ion-association complexes unite classical and non-classical theories for the biomimetic nucleation of calcium phosphate. Nat Commun. 2013;4:1507
Dorozhkin SV. Nanosized and nanocrystalline calcium orthophosphates. Acta Biomater. 2010;6:715–34.
Basim GB, Moudgil BM. Effect of soft agglomerates on CMP slurry performance. J Colloid Interf Sci. 2002;256:137–42.
Hannora AE, Ataya S. Structure and compression strength of hydroxyapatite/titania nanocomposites formed by high energy ball milling. J Alloy Compd. 2016;658:222–33.
Iyyappan E, Wilson P, Sheela K, Ramya R. Role of triton X-100 and hydrothermal treatment on the morphological features of nanoporous hydroxyapatite nanorods. Mat Sci Eng: C. 2016;63:554–62.
Schnitzler V, Fayon F, Despas C, Khairoun I, Mellier C, Rouillon T, Massiot D, Walcarius A, Janvier P, Gauthier O, Montavon G, Bouler JM, Bujoli B. Investigation of alendronate-doped apatitic cements as a potential technology for the prevention of osteoporotic hip fractures: critical influence of the drug introduction mode on the in vitro cement properties. Acta Biomater. 2011;7:759–70.
Nancollas GH, Tang R, Phipps RJ, Henneman Z, Gulde S, Wu W, Mangood A, Russell RGG, Ebetino FH. Novel insights into actions of bisphosphonates on bone: differences in interactions with hydroxyapatite. Bone. 2006;38:617–27.
Robinson J, Cukrowski I, Marques HM. Modelling the interaction of several bisphosphonates with hydroxyapatite using the generalised AMBER force field. J Mol Struc. 2006;825:134–42.
Pascaud P, Errassifi F, Brouillet F, Sarda S, Barroug A, Legrouri A, Rey C. Adsorption on apatitic calcium phosphates for drug delivery: interaction with bisphosphonate molecules. J Mater Sci: Mater Med. 2014;25:2373–81.
Acknowledgements
The present study was supported by the National Basic Research Program of China (973 Program, 2012CB933602), the National Natural Science Foundation of China (51372210, 50975239), the Research Fund for the Doctoral Program of Higher Education of China (20130184110023), the Basic Research Foundation Key Project of Sichuan Province (2016JY0011), and the Fundamental Research Funds for the Central Universities (2682016YXZT11).
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Gao, X., Dai, C., Liu, W. et al. High-scale yield of nano hydroxyapatite through combination of mechanical activation and chemical dispersion. J Mater Sci: Mater Med 28, 83 (2017). https://doi.org/10.1007/s10856-017-5892-9
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DOI: https://doi.org/10.1007/s10856-017-5892-9