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Assessment of bone regeneration of a tissue-engineered bone complex using human dental pulp stem cells/poly(ε-caprolactone)-biphasic calcium phosphate scaffold constructs in rabbit calvarial defects

  • Biocompatibility Studies
  • Original Research
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Abstract

The objective of the present study was to investigate the effect of a fabricated combination of poly-ɛ-caprolactone (PCL)–biphasic calcium phosphate (BCP) with the modified melt stretching and multilayer deposition (mMSMD) technique on human dental pulp stem cell (hDPSC) differentiation to be osteogenic like cells for bone regeneration of calvarial defects in rabbit models. hDPSCs extracted from human third molars were seeded onto mMSMD PCL-BCP scaffolds and the osteogenic gene expression was tested prior to implantation in vivo. Two standardized 11 mm in diameter circular calvarial defects were created in 18 adult male New Zealand white rabbits. The rabbits were divided into 4 groups: (1) hDPSCs seeded in mMSMD PCL-BCP scaffolds; (2) mMSMD PCL-BCP scaffolds alone, (3) empty defects and (4) autogenous bone (n = 3 site/time point/groups). After two, four and eight weeks after the operation, the specimens were harvested for micro-CT including histological and histomorphometric analysis. The explicit results presented an interesting view of the bioengineered constructs of hDPSCs in PCL-BCP scaffolds that increased the newly formed bone compared to the empty defect and scaffold alone groups. The results demonstrated that hDPSCs combined with mMSMD PCL-BCP scaffolds may be an augmentation material for bony defect.

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Acknowledgements

Our sincere gratitude goes to Associate Professor Premjit Arpornmaeklong for her expert advice and help. We would like to thank Mr. Jakchai Jantaramano for technical assistance and Mrs. Somporn Sretrirutchai for assistance in flow cytometry. We are also grateful for the financial support provided by a grant from the Faculty of Graduate Studies, Prince of Songkla University, Hat Yai, Songkhla, Thailand.

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Authors and Affiliations

  1. Department of Preventive Dentistry, Faculty of Dentistry, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand

    Natkrita Wongsupa & Suttatip Kamolmattayakul

  2. Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand

    Thongchai Nuntanaranont & Nuttawut Thuaksuban

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  1. Natkrita Wongsupa
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  2. Thongchai Nuntanaranont
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Correspondence to Thongchai Nuntanaranont.

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Wongsupa, N., Nuntanaranont, T., Kamolmattayakul, S. et al. Assessment of bone regeneration of a tissue-engineered bone complex using human dental pulp stem cells/poly(ε-caprolactone)-biphasic calcium phosphate scaffold constructs in rabbit calvarial defects. J Mater Sci: Mater Med 28, 77 (2017). https://doi.org/10.1007/s10856-017-5883-x

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