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In vitro cyto-biocompatibility study of thin-film transistors substrates using an organotypic culture method


Thin-Film-Transistors Liquid-Crystal Display has become a standard in the field of displays. However, the structure of these devices presents interest not only in that field, but also for biomedical applications. One of the key components, called here TFT substrate, is a glass substrate with a dense and large array of thousands of transparent micro-electrodes that can be considered as a large scale multi-electrode array(s). Multi-electrode array(s) are widely used for in vitro electrical investigations on neurons and brain, allowing excitation, registration, and recording of their activity. However, the range of application of conventional multi-electrode array(s) is usually limited to some tens of cells in a homogeneous cell culture, because of a small area, small number and a low density of the micro-electrodes. TFT substrates do not have these limitations and the authors are currently studying the possibility to use TFT substrates as new tools for in vitro electrical investigation on tissues and organoids. In this respect, experiments to determine the cyto-biocompatibility of TFT substrates with tissues were conducted and are presented in this study. The investigation was performed using an organotypic culture method with explants of brain and liver tissues of chick embryos. The results in term of morphology, cell migration, cell density and adhesion were compared with the results from Thermanox®, a conventional plastic for cell culture, and with polydimethylsiloxane, a hydrophobic silicone. The results with TFT substrates showed similar results as for the Thermanox®, despite the TFT hydrophobicity. TFT substrates have a weak cell adhesion and promote cell migration similarly to Thermanox®. It could be concluded that the TFT substrates are cyto-biocompatible with the two studied organs.

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  1. Gross GW, Rhoades BK, Azzazy HME, Wu MC. The use of neuronal networks on multi-electrode array(s) as biosensors. Biosens Bioelectron. 1995;10:553–67.

    Article  Google Scholar 

  2. Chopra KL, Major S, Pandya DK. Transparent conductors—a status review. Thin Solid Film. 1983;102(1):1–46.

    Article  Google Scholar 

  3. Ballini M, Muller J, Livi P, Yihui C, Frey U, Stettler A, Shadmani A, Viswam V, Lloyd Jones I, Jackel D, Radivojevic M, Lewandowska MK, Wei G, Fiscella M, Bakkum DJ, Heer F, Hierlemann A. A 1024-channel CMOS microelectrode array with 26,400 electrodes for recording and stimulation of electrogenic cells in vitro. IEEE J Solid-St Circ. 2014;49(11):2705–19.

    Article  Google Scholar 

  4. Kuo Y. Thin film transistor technology—past, present, and future. Electrochem Soc Interf. 2013;22(1):55–61.

  5. Sterling J-D, Chen C, Nadim A. Method, apparatus and article for microfluidic control via electrowetting, for chemical, biochemical and biological assays and the like. Patent US 2004/0231987 A1 2004.

  6. Tixier-Mita A, Ségard B-D, Kim Y-J, Matsunaga Y, Fujita H and Toshiyoshi H. TFT Display panel technology as a base for biological cells electrical manipulation—Application to dielectrophoresis. The 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS’2015, 18–22 January 2015; Estoril, Portugal.

  7. Wolff E, Haffen K. Sur une méthode de culture d’organes embryonnaires in vitro. Tex Rep Biol Med. 1952;10:463–72.

    Google Scholar 

  8. Sigot-Luizard MF, Lanfranchi M, Duval JL, Benslimane S, Sigot M, Guidoin RG, King MW. The cyto-compatibility of compound polyester-protein surfaces using an in vitro technique. In Vitro Cell Dev B. 1986;22:234

    Article  Google Scholar 

  9. Duval JL, Letort M, Sigot-Luizard MF. Comparative assessment of cell/substratum static adhesion using an in vitro organ culture method and computerized analysis system. Biomaterials. 1988;9:155–61.

    Article  Google Scholar 

  10. Duval JL, Dinis T, Vidal G, Vigneron P, Kaplan DL, Egles C. Organotypic culture to assess cell adhesion, growth and alignment of different organs on silk fibroin. J Tissue Eng Regen Med. 2014. doi:10.1002/term.1916

    Google Scholar 

  11. Leclerc E, Corlu A, Griscom L, Baudoin R, Legallais C. Guidance of liver and kidney organotypic cultures inside rectangular silicone microchannels. Biomaterials. 2006;27:4109–19.

    Article  Google Scholar 

  12. Anderson JR, Chiu DT, McDonald JC, Jackman RJ, Cherniavskaya O, Wu H, Whitesides S, Whitesides GM. Fabrication of topologically complex three-dimensional microfluidic systems in PDMS by rapid prototyping. Anal Chem. 2000;72:3158–64.

    Article  Google Scholar 

  13. Charati SG, Stern SA. Diffusion of gases in silicone polymer: molecular dynamics simulations. Macromolecules. 1998;31:5529–35.

    Article  Google Scholar 

  14. Fujii T. PDMS-based microfluidic devices for biomedical applications. Microelectron Eng. 2002;61-62:907–14.

    Article  Google Scholar 

  15. Churaev NV. Contact Angles and surface forces. Adv Colloid Interface Sci. 1995;58:87–118.

    Article  Google Scholar 

  16. Leclerc E, Duval J-L, Pezron I, Nadaud F. Behaviors of liver and kidney explants from chicken embryos inside plasma treated PDMS microchannel. Mat Sci Eng C. 2009;29:861–68.

    Article  Google Scholar 

  17. Frazer RQ, Byron RT, Osborne PB, West KP. PMMA: an essential material in medicine and dentistry. J Long Term Eff Med Implants. 2005;15:629–39.

    Article  Google Scholar 

  18. Hwang IT, Ahn MY, Jung CH, Choi JH, Shin K. Micropatterning of mammalian cells on indium tin oxide substrates using ion implantation. J Biomed Nanotechnol. 2013;9:819–24.

    Article  Google Scholar 

  19. Selvakumaran J, Hughes MP, Keddie JL, Ewins DJ. Assessing biocompatibility of materials for implantable microelectrodes using cytotoxicity and protein adsorption studies. Microtechnologies in Medicine & Biology 2nd Annual International IEEE-EMB. 2002; pp. 261–4

  20. Leclerc E, Corlu A, Griscom L, Baudoin R, Legallais C. Guidance of liver and kidney organotypic cultures inside rectangular silicone microchannels. Biomaterials. 2006;27:4109–19.

    Article  Google Scholar 

  21. Leclerc E, Duval JL, Griscom L, Baudoin R, Legallais C. Selective control of liver and kidney cells migration during organotypic co-cultures inside fibronectin coated rectangular silicone microchannels. Biomaterials. 2007;28:1820–29.

    Article  Google Scholar 

  22. Leclerc E, Duval JL, Jalabert L. Comparison of the migration of liver and kidney explants inside trapezoidal PDMS microchannels. Mater Sci Eng C. 2010;30:1190–6.

    Article  Google Scholar 

  23. Dalton BA, Walboomers XF, Dziegielewski M, Evans MD, Taylor S, Jansen JA, Steele JG. Modulation of epithelial tissue and cell migration by microgrooves. J Biomed Mater Res. 2001;56:195–207.

    Article  Google Scholar 

  24. Anderson AS, Olsson P, Lidberg U, Sutherland D. The effects of continuous and discontinuous groove edges on cell shape and alignment. Exp Cell Res. 2003;288:177–88.

    Article  Google Scholar 

  25. Hamilton DW, Wong KS, Brunette DM. Microfabricated discontinuous-edge surface topographies influence osteoblast adhesion, migration, cytoskeletal organization, and proliferation and enhance matrix and mineral deposition in vitro. Calcif Tissue Int. 2006;78:314–25.

    Article  Google Scholar 

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Correspondence to Agnès Tixier-Mita.

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Leclerc, E., Duval, JL., Egles, C. et al. In vitro cyto-biocompatibility study of thin-film transistors substrates using an organotypic culture method. J Mater Sci: Mater Med 28, 4 (2017).

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