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Bacterial and fungal biofilm formation on anodized titanium alloys with fluorine

  • Clinical Applications of Biomaterials
  • Original Research
  • Published:
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Abstract

Orthopaedic device-related infections are closely linked to biofilm formation on the surfaces of these devices. Several modified titanium (Ti-6Al-4V) surfaces doped with fluorine were studied in order to evaluate the influence of these modifications on biofilm formation by Gram-positive and Gram-negative bacteria as well as a yeast. The biofilm studies were performed according to the standard test method approved by ASTM (Designation: E2196-12) using the Rotating Disk Reactor. Four types of Ti-6Al-4V samples were tested; chemically polished (CP), two types of nanostructures containing fluorine, nanoporous (NP) and nanotubular (NT), and non-nanostructured fluorine containing samples (fluoride barrier layers, FBL). Different species of Gram-positive cocci, (Staphylococcus aureus and epidermidis), Gram-negative rods (Escherichia coli, Pseudomonas aeruginosa), and a yeast (Candida albicans) were studied. For one of the Gram-positive (S. epidermidis) and one of the Gram-negative (E. coli) species a statistically-significant decrease in biofilm accumulation for NP and NT samples was found when compared with the biofilm accumulation on CP samples. The results suggest an effect of the modified materials on the biofilm formation.

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Acknowledgments

This work was funded by the following grants from the Spanish MINECO (MAT2013-48224-C2-2-R and MAT2013-48224-C2-1-R). Pérez-Jorge C wishes to thank J William Costerton and the Medical Biofilm Laboratory at the Center for Biofilm Engineering in Montana, USA, for the opportunity to be a visiting researcher and the National Spanish Society of Infection Diseases and Microbiology (SEIMC) for funding with a Mobility grant 2012. Hernández-López J.M. is receptor of the JAE-predoctoral grant funded by the CSIC.

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Authors and Affiliations

  1. Department of Clinical Microbiology, IIS-Fundación Jiménez Díaz, Av. Reyes Católicos 2, 28040-, Madrid, Spain

    Concepcion Perez-Jorge & Jaime Esteban

  2. Department of Surface Engineering Corrosion and Durability, National Centre for Metallurgical Research, (CENIM-CSIC) Avda. Gregorio del Amo 8, 28040, Madrid, Spain

    Maria-Angeles Arenas, Ana Conde, Juan-Manuel Hernández-Lopez & Juan-Jose de Damborenea

  3. Center for Biofilm Engineering, Montana State University, 366 EPS Building. P.O. Box 173980, Bozeman, MT, 59717-3980, USA

    Steve Fisher & Garth James

  4. Department of Microbiology and Molecular Genetics, Michigan State University, 5180 Biomedical and Physical Sciences, East Lansing, MI, 48824, USA

    Alessandra M. Agostinho Hunt

Authors
  1. Concepcion Perez-Jorge
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  2. Maria-Angeles Arenas
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  3. Ana Conde
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  4. Juan-Manuel Hernández-Lopez
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  5. Juan-Jose de Damborenea
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  7. Alessandra M. Agostinho Hunt
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  8. Jaime Esteban
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Correspondence to Jaime Esteban.

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Perez-Jorge, C., Arenas, MA., Conde, A. et al. Bacterial and fungal biofilm formation on anodized titanium alloys with fluorine. J Mater Sci: Mater Med 28, 8 (2017). https://doi.org/10.1007/s10856-016-5811-5

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  • DOI: https://doi.org/10.1007/s10856-016-5811-5

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